Membraneless organelles and liquid-liquid phase separation – methods for their characterisation

Autor:	Tarczewska A; Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław. aneta.tarczewska@pwr.edu.pl., Wycisk K; Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław. krzysztof.wycisk@pwr.edu.pl., Sozańska N; Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław. 225423@student.pwr.edu.pl., Ożyhar A; Katedra Biochemii, Biologii Molekularnej i Biotechnologii, Wydział Chemiczny, Politechnika Wrocławska, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław. andrzej.ozyhar@pwr.edu.pl.
Jazyk:	polština
Zdroj:	Postepy biochemii [Postepy Biochem] 2020 Jun 27; Vol. 66 (2), pp. 111-124. Date of Electronic Publication: 2020 Jun 27.
DOI:	10.18388/pb.2020_330
Abstrakt:	Membraneless organelles (MLOs) are a large group of intracellular compartments formed during various stages of a cell life. They are important subcellular structures which enable a cell performance of vital physiological processes including stress response. MLOs can be found in cytoplasm and organelles that are sealed by lipidic membrane, mainly in nucleus. They are formed by the thermodynamically driven liquid-liquid phase separation (LLPS). MLOs contain proteins possessing intrinsically disordered regions (IDRs) which together with RNA spontaneously phase separate from the surrounding milieu. This paper presents information on the biophysical basses of the formation and functionality of MLOs. It also discusses a range of experimental techniques that can be applied in biochemical and biological studies of these sub-cellular structures.
Databáze:	MEDLINE
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