EO771, the first luminal B mammary cancer cell line from C57BL/6 mice.

Autor: Le Naour A; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Koffi Y; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Diab M; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Le Guennec D; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Rougé S; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Aldekwer S; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Goncalves-Mendes N; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Talvas J; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Farges MC; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Caldefie-Chezet F; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France., Vasson MP; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France.; Department of Nutrition, Gabriel Montpied University Hospital, Jean Perrin Cancer Centre, 58 rue Montalembert, 63011 Clermont-Ferrand, France., Rossary A; Human Nutrition Unit, ECREIN team, UMR 1019, University of Clermont Auvergne, INRAE, CRNH-Auvergne, TSA 50400, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France.
Jazyk: angličtina
Zdroj: Cancer cell international [Cancer Cell Int] 2020 Jul 20; Vol. 20, pp. 328. Date of Electronic Publication: 2020 Jul 20 (Print Publication: 2020).
DOI: 10.1186/s12935-020-01418-1
Abstrakt: Background: Despite decades of therapeutic trials, effective diagnosis, many drugs available and numerous studies on breast cancer, it remains the deadliest cancer in women. In order to choose the most appropriate treatment and to understand the prognosis of the patients, breast cancer is divided into different subtypes using a molecular classification. Just as there remains a need to discover new effective therapies, models to test them are also required.
Methods: The EO771 (also named E0771 or EO 771) murine mammary cancer cell line was originally isolated from a spontaneous tumour in C57BL/6 mouse. Although frequently used, this cell line remains poorly characterized. Therefore, the EO771 phenotype was investigated. The phenotype was compared to that of MCF-7 cells, known to be of luminal A subtype and to express estrogen receptors, as well as MDA-MB-231 cells, which are triple negative. Their sensitivity to hormonal treatment was evaluated by viability tests.
Results: The EO771 were estrogen receptor α negative, estrogen receptor β positive, progesterone receptor positive and ErbB2 positive. This phenotype was associated with a sensitivity to anti-estrogen treatments such as tamoxifen, 4-hydroxy-tamoxifen, endoxifen and fulvestrant.
Conclusions: On account of the numerous results published with the EO771 cell line, it is important to know its classification, to facilitate comparisons with corresponding types of tumours in patients. Transcriptomic and protein analysis of the EO771 cell line classified it within the luminal B subtype. Luminal B cancers correspond to one of the subtypes most frequently encountered in patients and associated with a poor prognosis.
Competing Interests: Competing interestsThe authors declare that they have no competing interests.
(© The Author(s) 2020.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje