Biosynthesis and Antimicrobial Activity of Pseudodesmin and Viscosinamide Cyclic Lipopeptides Produced by Pseudomonads Associated with the Cocoyam Rhizosphere.
Autor: | Oni FE; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.; Unit for Environmental Sciences and Management, Faculty of Natural and Agricultural Sciences, North-West University, Potchefstroom 2520, South Africa., Geudens N; NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Faculty of Science, Ghent University, Krijgslaan 281, B-9000 Gent, Belgium., Adiobo A; Jay PJ Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, Buea P. M. B 25, Cameroon., Omoboye OO; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.; Department of Microbiology, Obafemi Awolowo University, Ile-Ife 220005, Osun State, Nigeria., Enow EA; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium., Onyeka JT; Plant Pathology Unit, National Root Crops Research Institute (NRCRI), Umudike 440001, Abia State, Nigeria., Salami AE; Department of Crop, Horticulture and Landscape Design, Faculty of Agriculture, Ekiti State University (EKSU), Ado-Ekiti 360211, Nigeria., De Mot R; Centre of Microbial and Plant Genetics, Faculty of Bioscience Engineering, KU Leuven, 3001 Heverlee, Belgium., Martins JC; NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry, Faculty of Science, Ghent University, Krijgslaan 281, B-9000 Gent, Belgium., Höfte M; Laboratory of Phytopathology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. |
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Jazyk: | angličtina |
Zdroj: | Microorganisms [Microorganisms] 2020 Jul 20; Vol. 8 (7). Date of Electronic Publication: 2020 Jul 20. |
DOI: | 10.3390/microorganisms8071079 |
Abstrakt: | Pseudomonas cyclic lipopeptides (CLPs) are encoded non-ribosomally by biosynthetic gene clusters (BGCs) and possess diverse biological activities. In this study, we conducted chemical structure and BGC analyses with antimicrobial activity assays for two CLPs produced by Pseudomonas strains isolated from the cocoyam rhizosphere in Cameroon and Nigeria. LC-MS and NMR analyses showed that the Pseudomonas sp. COR52 and A2W4.9 produce pseudodesmin and viscosinamide, respectively. These CLPs belong to the Viscosin group characterized by a nonapeptidic moiety with a 7-membered macrocycle. Similar to other Viscosin-group CLPs, the initiatory non-ribosomal peptide synthetase (NRPS) gene of the viscosinamide BGC is situated remotely from the other two NRPS genes. In contrast, the pseudodesmin genes are all clustered in a single genomic locus. Nano- to micromolar levels of pseudodesmin and viscosinamide led to the hyphal distortion and/or disintegration of Rhizoctonia solani AG2-2 and Pythium myriotylum CMR1, whereas similar levels of White Line-Inducing Principle (WLIP), another member of the Viscosin group, resulted in complete lysis of both soil-borne phytopathogens. In addition to the identification of the biosynthetic genes of these two CLPs and the demonstration of their interaction with soil-borne pathogens, this study provides further insights regarding evolutionary divergence within the Viscosin group. |
Databáze: | MEDLINE |
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