Influence of LGALS3 and PNPLA3 genes in non-alcoholic steatohepatitis (NASH) in patients undergone bariatric surgery.

Autor: Azevedo Foinquinos G; School of Medical Sciences, University of Pernambuco, Brazil., Azevedo Acioli ME; Aggeu Magalhães Institute - IAM- FIOCRUZ-PE, Brazil., Santana Cavalcanti AH; Aggeu Magalhães Institute - IAM- FIOCRUZ-PE, Brazil., Barbosa Junior WL; Aggeu Magalhães Institute - IAM- FIOCRUZ-PE, Brazil., Lima RE; Aggeu Magalhães Institute - IAM- FIOCRUZ-PE, Brazil., Juca NT; Studio of Surgical Pathology - SPAC, Brazil., de Azevedo Foinquinos RC; Studio of Surgical Pathology - SPAC, Brazil., Rocha da Cruz C; School of Medical Sciences, University of Pernambuco, Brazil., Fernandez Pereira FM; School of Medical Sciences, University of Pernambuco, Brazil., de Carvalho SR; School of Medical Sciences, University of Pernambuco, Brazil., de Mendonça Belmont TF; School of Medical Sciences, University of Pernambuco, Brazil., Vasconcelos LRS; School of Medical Sciences, University of Pernambuco, Brazil; Aggeu Magalhães Institute - IAM- FIOCRUZ-PE, Brazil; Institute of Liver and Transplant of Pernambuco, Brazil. Electronic address: luydson.vasconcelos@cpqam.fiocruz.br., Beltrão Pereira LMM; School of Medical Sciences, University of Pernambuco, Brazil; Institute of Liver and Transplant of Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Obesity research & clinical practice [Obes Res Clin Pract] 2020 Jul - Aug; Vol. 14 (4), pp. 326-332. Date of Electronic Publication: 2020 Jul 18.
DOI: 10.1016/j.orcp.2020.07.004
Abstrakt: Aim: This study evaluated the genesPNPLA3 and LGALS3 in patients who have undergone bariatric surgery.
Methods: Individuals with NAFLD and NASH were evaluated, the DNA was extracted from total blood for genotyping of rs4644, rs4652 from LGALS3 and rs738409 from PNPLA3 genes, the total RNA was obtained from liver biopsy. For the detection of the molecular targets, real-time PCR through Taqman probes was used.
Results: From a total of 46 collected patients, of those 21 (456%) were included as NASH and 25 (544%) as steatosis group. This groups showed significant difference to aspartate aminotransferase (AST), alanine aminotransferase (ALT) and Glutamyl transpeptidase (GGT) (p = 0.0108, p = 0.0090 and p = 0.0044). Regarding to gene expression in studied groups, hepatic steatosis vs NASH, we observed a higher expression of the LGALS3 gene in NASH (p = 0.0273). In addition, patients with C allele in homozygous for rs4644 and rs4652 of LGALS3 gene had higher expression, in NASH group (p = 0.0500 and p = 0.0242, respectively), furthermore for rs4644 both alleles in homozygous showed higher expression (AA/CC vs AC) (p = 0.0500), when analyzed PNPLA3 rs738409, NASH patients with G allele in homozygous had higher expression (p = 0.0494).
Conclusions: Therefore, an increased expression of the LGALS3 gene in patients with NASH may be important in the etiopathogenesis of the disease, as well as the presence of rs4652 and rs4644 SNPs in the regulation of transcriptional levels of the gene in patients with NAFLD and NASH.
(Copyright © 2020. Published by Elsevier Ltd.)
Databáze: MEDLINE
Externí odkaz: