Production of transgenic Eucalyptus grandis x E. urophylla using the sonication-assisted Agrobacterium transformation (SAAT) system.

Autor: González ER; Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Andrade A; Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Bertolo AL; Departamento de Genética, EsDepartamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Lacerda GC; Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Carneiro RT; Departamento de GenéticDepartamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Defávari VAP; Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Labate MTV; Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil., Labate CA; Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Av. Pádua Dias 11, CP 83, CEP 13400970, Piracicaba-SP, Brazil.Corresponding author; email: calabate@carpa.ciagri.usp.br.
Jazyk: angličtina
Zdroj: Functional plant biology : FPB [Funct Plant Biol] 2002 Jan; Vol. 29 (1), pp. 97-102.
DOI: 10.1071/PP01142
Abstrakt: A method for genetic transformation of germinating seeds and seedlings of Eucalyptus grandis × E. urophyllais described using the sonication-assisted Agrobacterium-mediated transformation (SAAT) system. Seeds germinated for 2 d, and 15-d-old seedlings, sonicated for 30 s, had the highest percentage of β-glucuronidase (GUS) transient expression (21.7 and 37.4%, respectively). Pre-sonication greatly enhanced the efficiency of transformation. The differential transformation of tissues was also investigated, with seeds imbibed for 2 d having over 90% of the blue sectors localised in cotyledons and in the intersection of the hypocotyls and roots, whereas in 5-d-old seedlings, 70% of GUS activity was detected in cotyledons. However, 15-17-d-old seedlings had around 60% of transformed sectors localised in the first pair of leaves. The efficiency of the method was also assessed using a chimeric construct containing the Lhcb1*2 gene of the 28 kDa chlorophyll a/b binding pea protein from the LHCII antenna. Four stable transformants were confirmed by genomic blotting.
Databáze: MEDLINE
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