Molecular detection of Histoplasma capsulatum in bats of the Amazon biome in Pará state, Brazil.

Autor: da Silva JA; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Scofield A; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Barros FN; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., de Farias DM; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Riet-Correa G; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Bezerra Júnior PS; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Santos TFS; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Tavares GSF; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil., Trevelin LC; Instituto Tecnológico Vale Desenvolvimento Sustentável, Belém, Brazil., da Paz GS; Departamento de Higiene Veterinária e Saúde Pública, Campus de Botucatu, Universidade Estadual Paulista Julio de Mesquita Filho, Botucatu, Brazil., Cerqueira VD; Faculty of Veterinary Medicine, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2021 Mar; Vol. 68 (2), pp. 758-766. Date of Electronic Publication: 2020 Aug 04.
DOI: 10.1111/tbed.13740
Abstrakt: Histoplasma capsulatum, the fungus causing histoplasmosis, has a strong impact on public health. Histoplasmosis is one of the most prevalent systemic mycoses in the Americas and occurs in several mammalian species. Bats are important in the epidemiological cycle of histoplasmosis because they disseminate the fungus throughout the environment. The aim of the present study was to investigate natural H. capsulatum infection in bats located in forested areas, which have undergone anthropogenic perturbations, as well as in the urban areas of the state of Pará. Twenty-two species of bats were captured in 18 municipalities of Pará; the samples obtained from these animals were subjected to nested PCR for amplification of H. capsulatum DNA. The HCI/HCII and HCIII/HCIV primers were used, and the final 210-pb fragment was amplified. Of the 100 bats analysed, two were confirmed to be positive for H. capsulatum. Samples amplified by nested PCR were sequenced and found to share identity and have 100% match with H. capsulatum DNA. H. capsulatum was detected in the area of study: the state of Pará has a wide diversity of bat species, and the region under investigation is situated in the north of the state, which suffers the most severe environmental and climatic changes. Therefore, it is essential to elucidate the distribution of H. capsulatum hosts in this region to facilitate the implementation of effective disease surveillance.
(© 2020 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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