Panax ginseng metabolite (GIM-1) modulates the effects of monobutyl phthalate (MBP) on the GPR30/GPER1 canonical pathway in human Sertoli cells.

Autor: de Freitas ATAG; São Paulo State University (UNESP), Institute of Biosciences, Department of Structural and Functional Biology, Botucatu, SP, 18618-689, Brazil., Pinho CF; São Paulo State University (UNESP), Institute of Biosciences, Department of Structural and Functional Biology, Botucatu, SP, 18618-689, Brazil., Aquino AM; São Paulo State University (UNESP), Institute of Biosciences, Department of Structural and Functional Biology, Botucatu, SP, 18618-689, Brazil., Domeniconi RF; São Paulo State University (UNESP), Institute of Biosciences, Department of Structural and Functional Biology, Botucatu, SP, 18618-689, Brazil., Justulin LA; São Paulo State University (UNESP), Institute of Biosciences, Department of Structural and Functional Biology, Botucatu, SP, 18618-689, Brazil., Scarano WR; São Paulo State University (UNESP), Institute of Biosciences, Department of Structural and Functional Biology, Botucatu, SP, 18618-689, Brazil. Electronic address: wellerson.scarano@unesp.br.
Jazyk: angličtina
Zdroj: Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2020 Sep; Vol. 96, pp. 209-215. Date of Electronic Publication: 2020 Jul 16.
DOI: 10.1016/j.reprotox.2020.07.004
Abstrakt: This study was performed to evaluate the effect of monobutyl phthalate (MBP) on GPR30-activated pathways in Sertoli cells. Additionally, we tested if GIM-1 (Panax ginseng metabolite) modulates MBP action. Human Sertoli cells (HSeC lineage) were exposed to MBP and/or GIM-1 for 30 min, 1, 12, and 48 h. Four experimental treatments were performed: control (DEMEM/F12 medium), MBP, GIM-1, and MBP + GIM-1. The results indicate that MBP activates GPR30, PKA, Src, EGFR, and the ERK1/2 proteins, while GIM-1 inhibits PKA, Src, ERK1/2, and the AKT pathway. MBP also enhances Cofilin expression, decreasing F-actin intensity on the cell surface in a short time. The combined exposure demonstrated a functional antagonism between compounds. Collectively, these data show that MBP activates GPR30 in Sertoli cells, and GIM-1 modulates this response, playing a protective role in Sertoli cells exposed to MBP.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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