Contaminated water confirmed as source of infection by bioassay in an outbreak of toxoplasmosis in South Brazil.

Autor: Minuzzi CE; Universidade Federal de Santa Maria, Santa Maria, Brazil., Fernandes FD; Universidade Federal de Santa Maria, Santa Maria, Brazil., Portella LP; Universidade Federal de Santa Maria, Santa Maria, Brazil., Bräunig P; Universidade Federal de Santa Maria, Santa Maria, Brazil., Sturza DAF; Instituto Samitec, Santa Maria, Brazil., Giacomini L; Instituto Samitec, Santa Maria, Brazil., Salvagni E; Secretaria da Saúde do estado do Rio Grande do Sul, Santa Maria, Brazil., Ribeiro JDS; Secretaria da Saúde do estado do Rio Grande do Sul, Santa Maria, Brazil., Silva CR; CIEVS/Episus e Coordenação Geral de Laboratórios, SVS, Ministério da Saúde, Brasília, Brazil., Difante CM; Superintendência de Vigilância em Saúde, Secretaria Municipal de Saúde, Santa Maria, Brazil., Farinha LB; Vigilância Epidemiológica 4ª Regional de Saúde/Secretaria da Saúde/RS, Santa Maria, Brazil., Menegolla IA; CIEVS, DAT, CEVS, Secretaria da Saúde/RS, Santa Maria, Brazil., Gehrke G; Universidade Federal de Santa Maria, Santa Maria, Brazil., Dilkin P; Instituto Samitec, Santa Maria, Brazil., Sangioni LA; Universidade Federal de Santa Maria, Santa Maria, Brazil., Mallmann CA; Instituto Samitec, Santa Maria, Brazil., Vogel FSF; Universidade Federal de Santa Maria, Santa Maria, Brazil.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2021 Mar; Vol. 68 (2), pp. 767-772. Date of Electronic Publication: 2020 Aug 30.
DOI: 10.1111/tbed.13741
Abstrakt: The protozoan Toxoplasma gondii is a causative agent of toxoplasmosis, an important and widespread zoonotic disease. The transmission of this disease in humans includes ingestion of sporulated oocysts present in contaminated water or food. T. gondii oocysts are widely distributed and toxoplasmosis is considered a major food- and waterborne pathogen worldwide, making drinking water containing sporulated T. gondii oocysts a major source of contamination for people. In the first half of 2018, an unprecedented outbreak of toxoplasmosis was reported in the city of Santa Maria, southern Brazil. The temporal and spatial distribution of the cases strongly suggested a waterborne infection. Thus, the aim of this study was to investigate a possible involvement of treated water as a source of the outbreak. For this, piglets received potentially contaminated water ad libitum for 21 days and the infection was monitored by serology through IFAT and investigation of T. gondii DNA in tissues by PCR amplification of a 529 bp followed by mouse bioassays. All piglets receiving test water ad libitum for 21 days as well as positive controls seroconverted to T. gondii. T. gondii DNA was detected in 62.5% of the piglets that received test water. All mice inoculated with tissues from each positive piglet were PCR-positive. These results strongly indicated the presence of viable oocysts in the test water administered to the animals during the study.
(© 2020 Wiley-VCH GmbH.)
Databáze: MEDLINE
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