Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture.

Autor:	Chizhik AI; Third Institute of Physics - Biophysics, Georg August University, Göttingen, Germany., Chizhik AM; Third Institute of Physics - Biophysics, Georg August University, Göttingen, Germany., Ruhlandt D; Third Institute of Physics - Biophysics, Georg August University, Göttingen, Germany., Pfaff J; Department of Molecular Biology, GZMB, University Medical Center of Göttingen, Georg August University, Göttingen, Germany., Karedla N; Third Institute of Physics - Biophysics, Georg August University, Göttingen, Germany., Gregor I; Third Institute of Physics - Biophysics, Georg August University, Göttingen, Germany., Kehlenbach RH; Department of Molecular Biology, GZMB, University Medical Center of Göttingen, Georg August University, Göttingen, Germany., Enderlein J; Third Institute of Physics - Biophysics, Georg August University, Göttingen, Germany. jenderl@gwdg.de.; Multiscale Bioimaging: from Molecular Machines to Networks of Ex-citable Cells (MBExC), Georg August University, Göttingen, Germany. jenderl@gwdg.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2175, pp. 33-45.
DOI:	10.1007/978-1-0716-0763-3_4
Abstrakt:	The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and control transport of macromolecules between the two compartments. Recently, it has been shown that the axial distance between the inner nuclear membrane and the cytoplasmic side of the NPC can be measured using dual-color metal-induced energy transfer (MIET). This chapter focuses on experimental aspects of this method and discusses the details of data analysis.
Databáze:	MEDLINE
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