Cupriavidus necator B-10646 growth and polyhydroxyalkanoates production on different plant oils.

Autor: Volova T; Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russian Federation., Sapozhnikova K; Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russian Federation., Zhila N; Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russian Federation; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, Krasnoyarsk 660036, Russian Federation. Electronic address: nzhila@mail.ru.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 01; Vol. 164, pp. 121-130. Date of Electronic Publication: 2020 Jul 14.
DOI: 10.1016/j.ijbiomac.2020.07.095
Abstrakt: The study addresses the growth of the wild-type strain Cupriavidus necator B-10646 and synthesis of polyhydroxyalkanoates by this strain on media containing plant oils with different compositions of fatty acids: palm, Siberian oilseed, and refined and unrefined sunflower seed oils. The study showed that the best carbon substrate was palm oil. Comparison of fatty acid compositions of the starting oils and unutilized residual substrates showed that C. necator B-10646 cells consumed the fatty acids from palm oil evenly while in experiments with other oils, they utilized polyenoic fatty acids first. Higher production parameters of the culture were obtained by preparation of emulsified oil medium using Tween 80 and sodium cocoyl glutamate as emulsifiers. All polyhydroxyalkanoate specimens were terpolymers that contained 3-hydroxybutyrate as the major component and minor amounts of 3-hydroxyvalerate (0.9-1.9 mol%) and 3-hydroxyhexanoate (0.5-1.1 mol%). Molecular weight of polyhydroxyalkanoate specimens depended on the type of plant oil and emulsifier.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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