[Expression of B7 Family Co-inhibitory Molecules in the Establishment of Pulmonary Regional Immunity].

Autor:	Xie YQ; The Vascular Remodeling and Developmental Defects Research Unit, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., Shi F; The Vascular Remodeling and Developmental Defects Research Unit, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China., Cao BR; Department of Basic Research, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China., Wang HJ; The Vascular Remodeling and Developmental Defects Research Unit, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China.; Chengdu Newgenegle Biotech Co. Ltd.,Chengdu 610041, China., Liu HM; The Vascular Remodeling and Developmental Defects Research Unit, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China.; Department of Pediatric Respiratory, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Jazyk:	čínština
Zdroj:	Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2018 Nov; Vol. 49 (6), pp. 886-890.
Abstrakt:	Objective: To identify the temporal-spatial expression of B7 family co-inhibitory molecules during lung development, and to explore the roles of B7 family co-inhibitory molecules in the developmental process of pulmonary regional immunity. Methods: The expression of B7 family co-inhibitory molecules (B7-1, B7-2, B7-H1, B7-DC) in different developmental stages of Rhesus monkey lungs were normalized and calculated by the reads per kilo-base of transcript per million mapped reads (RPKM) method. Immunohistochemical staining was performed to identify the localization and the protein of B7 family co-inhibitory molecules in different developmental phase (canalicular stage, cystic stage, alveolar stage) in mouse. Results: The expression of B7 family co-inhibitory molecules in rhesus monkey were increased during the prenatal period (cystic stage, alveolar stage), the expressions of B7-2 and B7-H1 mRNA were significantly increased in alveolar stage ( P <0.05). The results of immuno-histochemistry indicated that B7 family co-inhibitory molecules were mainly expressed in airway epithelial cells, and their protein levels were increased during the prenatal period. The expressions of B7-2, B7-H1 and B7-DC were significantly increased from canalicular stage ( P <0.05). The protein of B7-2 was higher in airway than that in bronchus ( P <0.05). Conclusions: B7 family co-inhibitory molecules are mainly expressed in airway epithelial cells, and the expressions are increased during the prenatal period, which suggests that B7 family co-inhibitory molecules are involved possibly in the development of pulmonary regional immunity. (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)
Databáze:	MEDLINE
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