Human B cell clonal expansion and convergent antibody responses to SARS-CoV-2.

Autor: Nielsen SCA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.; These authors contributed equally., Yang F; Department of Pathology, Stanford University, Stanford, CA 94305, USA.; These authors contributed equally., Jackson KJL; Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.; These authors contributed equally., Hoh RA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.; These authors contributed equally., Röltgen K; Department of Pathology, Stanford University, Stanford, CA 94305, USA., Stevens B; Department of Pathology, Stanford University, Stanford, CA 94305, USA., Lee JY; Department of Pathology, Stanford University, Stanford, CA 94305, USA., Rustagi A; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA., Rogers AJ; Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University, Stanford, CA 94305, USA., Powell AE; Stanford ChEM-H and Department of Biochemistry, Stanford University, Stanford, CA 94305, USA., Najeeb J; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA., Otrelo-Cardoso AR; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA., Yost KE; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA., Daniel B; Department of Pathology, Stanford University, Stanford, CA 94305, USA., Chang HY; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Satpathy AT; Department of Pathology, Stanford University, Stanford, CA 94305, USA., Jardetzky TS; Department of Structural Biology, Stanford University, Stanford, CA 94305, USA.; Sean N. Parker Center for Allergy and Asthma Research, Stanford, CA 94305, USA., Kim PS; Stanford ChEM-H and Department of Biochemistry, Stanford University, Stanford, CA 94305, USA.; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Wang TT; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA.; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.; Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA., Pinsky BA; Department of Pathology, Stanford University, Stanford, CA 94305, USA., Blish CA; Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University, Stanford, CA 94305, USA.; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Boyd SD; Department of Pathology, Stanford University, Stanford, CA 94305, USA.; Sean N. Parker Center for Allergy and Asthma Research, Stanford, CA 94305, USA.; Lead Contact.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2020 Jul 09. Date of Electronic Publication: 2020 Jul 09.
DOI: 10.1101/2020.07.08.194456
Abstrakt: During virus infection B cells are critical for the production of antibodies and protective immunity. Here we show that the human B cell compartment in patients with diagnostically confirmed SARS-CoV-2 and clinical COVID-19 is rapidly altered with the early recruitment of B cells expressing a limited subset of IGHV genes, progressing to a highly polyclonal response of B cells with broader IGHV gene usage and extensive class switching to IgG and IgA subclasses with limited somatic hypermutation in the initial weeks of infection. We identify extensive convergence of antibody sequences across SARS-CoV-2 patients, highlighting stereotyped naïve responses to this virus. Notably, sequence-based detection in COVID-19 patients of convergent B cell clonotypes previously reported in SARS-CoV infection predicts the presence of SARS-CoV/SARS-CoV-2 cross-reactive antibody titers specific for the receptor-binding domain. These findings offer molecular insights into shared features of human B cell responses to SARS-CoV-2 and other zoonotic spillover coronaviruses.
Databáze: MEDLINE