Fitness of Outer Membrane Vesicles From Komagataeibacter intermedius Is Altered Under the Impact of Simulated Mars-like Stressors Outside the International Space Station.

Autor:	Podolich O; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Kukharenko O; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Zaets I; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Orlovska I; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Palchykovska L; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Zaika L; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Sysoliatin S; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Zubova G; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine., Reva O; Centre for Bioinformatics and Computational Biology, University of Pretoria, Pretoria, South Africa., Galkin M; Palladin Institute of Biochemistry of NASU, Kyiv, Ukraine., Horid'ko T; Palladin Institute of Biochemistry of NASU, Kyiv, Ukraine., Kosiakova H; Palladin Institute of Biochemistry of NASU, Kyiv, Ukraine., Borisova T; Palladin Institute of Biochemistry of NASU, Kyiv, Ukraine., Kravchenko V; NanoMedTech LLC, Kyiv, Ukraine., Skoryk M; Department of Computer Science, Virginia Commonwealth University, Richmond, VA, United States., Kremenskoy M; NanoMedTech LLC, Kyiv, Ukraine., Ghosh P; Department of Computer Science, Virginia Commonwealth University, Richmond, VA, United States., Barh D; Centre for Genomics and Applied Gene Technology, Institute of Integrative Omics and Applied Biotechnology (IIOAB), Purba Medinipur, India., Góes-Neto A; Molecular and Computational Biology of Fungi Laboratory, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil., Azevedo V; Laboratory of Cellular and Molecular Genetics, Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil., de Vera JP; Institute of Planetary Research, German Aerospace Center, Berlin, Germany., Kozyrovska N; Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2020 Jun 26; Vol. 11, pp. 1268. Date of Electronic Publication: 2020 Jun 26 (Print Publication: 2020).
DOI:	10.3389/fmicb.2020.01268
Abstrakt:	Outer membrane vesicles (OMVs), produced by nonpathogenic Gram-negative bacteria, have potentially useful biotechnological applications in extraterrestrial extreme environments. However, their biological effects under the impact of various stressors have to be elucidated for safety reasons. In the spaceflight experiment, model biofilm kombucha microbial community (KMC) samples, in which Komagataeibacter intermedius was a dominant community-member, were exposed under simulated Martian factors (i.e., pressure, atmosphere, and UV-illumination) outside the International Space Station (ISS) for 1.5 years. In this study, we have determined that OMVs from post-flight K. intermedius displayed changes in membrane composition, depending on the location of the samples and some other factors. Membrane lipids such as sterols, fatty acids (FAs), and phospholipids (PLs) were modulated under the Mars-like stressors, and saturated FAs, as well as both short-chain saturated and trans FAs, appeared in the membranes of OMVs shed by both post-UV-illuminated and "dark" bacteria. The relative content of zwitterionic and anionic PLs changed, producing a change in surface properties of outer membranes, thereby resulting in a loss of interaction capability with polynucleotides. The changed composition of membranes promoted a bigger OMV size, which correlated with changes of OMV fitness. Biochemical characterization of the membrane-associated enzymes revealed an increase in their activity (DNAse, dehydrogenase) compared to wild type. Other functional membrane-associated capabilities of OMVs (e.g., proton accumulation, interaction with linear DNA, or synaptosomes) were also altered after exposure to the spaceflight stressors. Despite alterations in membranes, vesicles did not acquire endotoxicity, cytotoxicity, and neurotoxicity. Altogether, our results show that OMVs, originating from rationally selected nonpathogenic Gram-negative bacteria, can be considered as candidates in the design of postbiotics or edible mucosal vaccines for in situ production in extreme environment. Furthermore, these OMVs could also be used as promising delivery vectors for applications in Astromedicine. (Copyright © 2020 Podolich, Kukharenko, Zaets, Orlovska, Palchykovska, Zaika, Sysoliatin, Zubova, Reva, Galkin, Horid’ko, Kosiakova, Borisova, Kravchenko, Skoryk, Kremenskoy, Ghosh, Barh, Góes-Neto, Azevedo, de Vera and Kozyrovska.)
Databáze:	MEDLINE
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