Embryo yield and quality are associated with progestogen treatment during superovulation protocol in lactating Lacaune ewes.

Autor: Figueira LM; Universidade Federal de Lavras - Av. Doutor Sylvio Menicucci, 1001 - Kennedy, CEP 37200-900, Lavras, MG, Brazil; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil., Alves NG; Universidade Federal de Lavras - Av. Doutor Sylvio Menicucci, 1001 - Kennedy, CEP 37200-900, Lavras, MG, Brazil. Electronic address: nadja@ufla.br., Maia ALRES; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil., Souza-Fabjan JMG; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil., Batista RITP; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil., Morais MCDC; Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Vital Brazil, CEP 24220-000, Niterói, RJ, Brazil., Lima RR; Universidade Federal de Lavras - Av. Doutor Sylvio Menicucci, 1001 - Kennedy, CEP 37200-900, Lavras, MG, Brazil., Oliveira MEF; Universidade Estadual Paulista 'Júlio de Mesquita Filho, Via Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil., Fonseca JFD; Embrapa Caprinos e Ovinos, Estrada Sobral/ Groaíras, km 4, CP D10, CEP 62011-000, Sobral, CE, Brazil. Electronic address: jeferson.fonseca@embrapa.br.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2020 Oct 01; Vol. 155, pp. 132-138. Date of Electronic Publication: 2020 Jun 08.
DOI: 10.1016/j.theriogenology.2020.06.004
Abstrakt: This study assessed the effect of progestogen treatment length on ovarian parameters and embryo yield in superovulated Lacaune ewes collected by nonsurgical embryo recovery. Twenty-three lactating ewes were superovulated 30 d apart using a cross-over design. All ewes received 60 mg of MAP intravaginal sponges for 6 (G-6 group) or 9 (G-9 group) d. A total dose of 133 mg pFSH was given in six decreasing doses (twice a day) starting at 60 h before device removal. Ultrasound examination of the ovaries was performed at the first pFSH injection and one day before embryo recovery, which was performed 6-7 d after the onset of estrus. Embryo recovery was conducted only in ewes that expressed estrus and were mated. There was no difference (P > 0.05) in the total number of follicles between G-6 (15.7 ± 1.0) and G-9 (15.6 ± 0.8) at the time of the first pFSH treatment. The percentage of responding donors with ≥3 corpora lutea (CL; 78.2% [18/23] vs 69.5% [16/23]), mean (±SEM) CL number (7.0 ± 1.2 vs 8.1 ± 1.6), transcervical passage rate (94.4% [17/18] vs 83.3% [15/18], and ova/embryo recovery rate (54.5% [60/110] vs 68.0% [83/122]) were not different (P > 0.05) between the G-6 and G-9 groups. However, the mean number of viable embryos was lower (P < 0.05) in the G-6 group (1.8 ± 0.7) than in the G-9 group. (3.5 ± 1.1). In conclusion, treatment with an intravaginal MAP sponge for 9 d during a superovulation protocol is beneficial for viable embryo yield in Lacaune ewes out of the breeding season.
Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interests.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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