| Autor: |
García JFM; Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. de los Barrios #1, Los Reyes Iztacala, 54090, Tlalnepantla, State of Mexico, Mexico.; Licenciatura en Nutrición, Unidad Académica Profesional Acolman, Universidad Autónoma del Estado de Mexico, Acolman, Estado de Mexico, Mexico., Rojas L; Laboratorio Nacional de Servicios Experimentales, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN 2508, Zacatenco, 07360, Mexico City, Mexico., Zenteno E; Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico., Cruz CV; Centro de Investigaciones en Ciencias Microbiológicas, BUAP, Apdo. Postal 1622, 72560, Puebla, Puebla, Mexico., Abascal EN; Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Av. de los Barrios #1, Los Reyes Iztacala, 54090, Tlalnepantla, State of Mexico, Mexico. negretee@yahoo.com. |
| Abstrakt: |
Actinobacillus seminis is an autochthonous gram-negative bacterium that affects reproductive organs, causing epididymitis, low fertility, and occasional abortions in ovine and goats. The virulence factors and the pathogenicity mechanisms of A. seminis have not been clearly elucidated yet. In this work, biofilm production by A. seminis in in vitro assays is described and characterized. After 48-h incubation at 37 °C in trypticase soy broth, A. seminis formed biofilms containing an extracellular matrix comprised mainly of fibrillar material. Microaerophilia or the presence of calcium diminished biofilm formation in approximately 50% and 70%, respectively, but low iron concentrations increased it 40%. Through enzymatic digestion, it was found that proteins were the main component of these biofilms. Structural observations through scanning electron microscopy indicated the presence of a high amount of fibrillar material in which bacteria were immersed. Antibodies against different bacterial surface proteins, such as anti-biofilm matrix and anti-adhesin, diminished biofilm formation in 70% and 25%, respectively; whereas furanone C-30 and LED-209, compounds described as quorum-sensing inhibitors, completely inhibited biofilm formation. In conclusion, environmental conditions can influence strongly biofilm formation in A. seminis, and this could be an advantageous strategy that allows bacteria to persist inside a host. |
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