Tissue Factor-Targeted ImmunoPET Imaging and Radioimmunotherapy of Anaplastic Thyroid Cancer.

Autor: Wei W; Department of Nuclear Medicine Institute of Clinical Nuclear Medicine Renji Hospital, School of Medicine Shanghai Jiao Tong University State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute 1630 Dongfang Rd Shanghai 200127 China.; Departments of Radiology and Medical Physics University of Wisconsin-Madison Madison WI 53705 USA., Liu Q; Department of Nuclear Medicine Fudan University Shanghai Cancer Center Fudan University 270 Dongan Rd Shanghai 200032 China., Jiang D; Departments of Radiology and Medical Physics University of Wisconsin-Madison Madison WI 53705 USA., Zhao H; Department of Nuclear Medicine Institute of Clinical Nuclear Medicine Renji Hospital, School of Medicine Shanghai Jiao Tong University State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute 1630 Dongfang Rd Shanghai 200127 China., Kutyreff CJ; Departments of Radiology and Medical Physics University of Wisconsin-Madison Madison WI 53705 USA., Engle JW; Departments of Radiology and Medical Physics University of Wisconsin-Madison Madison WI 53705 USA., Liu J; Department of Nuclear Medicine Institute of Clinical Nuclear Medicine Renji Hospital, School of Medicine Shanghai Jiao Tong University State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute 1630 Dongfang Rd Shanghai 200127 China., Cai W; Departments of Radiology and Medical Physics University of Wisconsin-Madison Madison WI 53705 USA.; University of Wisconsin Carbone Cancer Center Madison WI 53705 USA.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2020 May 17; Vol. 7 (13), pp. 1903595. Date of Electronic Publication: 2020 May 17 (Print Publication: 2020).
DOI: 10.1002/advs.201903595
Abstrakt: Anaplastic thyroid cancer (ATC) is the most aggressive subtype of thyroid cancers with a dismal prognosis. It is aimed to explore a new biomarker and devise a marker-dependent theranostic pair for ATC. Flow cytometry is used to determine tissue factor (TF) expression in thyroid cancer cell lines. ALT-836, a TF-specific monoclonal antibody, is radiolabeled with 64 Cu to develop 64 Cu-NOTA-ALT-836. The diagnostic utility is assessed by immuno-positron emission tomography (immunoPET) imaging in ATC models. To facilitate total surgical removal of orthotopic ATCs, a near-infrared fluorescent imaging probe IRDye 800CW-ALT-836 is designed. As the therapeutic component, 131 I-ALT-836 is further developed and the radioimmunotherapy (RIT) efficacy of this agent is interrogated in orthotopic ATC models. The results demonstrate that TF is highly expressed on the ATC cell line THJ-16T. 64 Cu-NOTA-ALT-836 immunoPET imaging clearly delineates both subcutaneous and orthotopic ATCs, with a peak tumor uptake of 19.93 ± 2.17% ID per g ( n = 3) and 37.20 ± 1.71% ID per g ( n = 3), respectively. Fluorescent imaging with IRDye 800CW-ALT-836 facilitates the total resection of orthotopic ATCs. Moreover, 131 I-ALT-836 RIT prolongs the survival of ATC-bearing mice. Taken together, TF is a promising marker for ATC and successive use of 64 Cu-NOTA-ALT-836 and 131 I-ALT-836 can realize precise management of ATC.
Competing Interests: The authors declare no conflict of interest.
(© 2020 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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