Safety and delivery efficiency of a photodynamic treatment of the lungs using indocyanine green and extracorporeal near infrared illumination.

Autor: Kassab G; São Carlos Institute of Physics, University of São Paulo, São Carlos, Brazil., Cheburkanov V; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA., Willis J; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA., Moule MG; Department of Microbial Pathogenesis and Immunology, Texas A&M University College of Medicine, Bryan, Texas, USA., Kurachi C; São Carlos Institute of Physics, University of São Paulo, São Carlos, Brazil., Yakovlev V; Department of Biomedical Engineering, Texas A&M University, College Station, Texas, USA., Cirillo JD; Department of Microbial Pathogenesis and Immunology, Texas A&M University College of Medicine, Bryan, Texas, USA., Bagnato VS; São Carlos Institute of Physics, University of São Paulo, São Carlos, Brazil.; Hagler Institute for Advances Studies, Texas A&M University, College Station, Texas, USA.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2020 Oct; Vol. 13 (10), pp. e202000176. Date of Electronic Publication: 2020 Aug 12.
DOI: 10.1002/jbio.202000176
Abstrakt: Photodynamic inactivation (PDI) is a promising alternative for combating infections caused by antimicrobial resistant bacteria. Pneumonias are among the most worrisome infections because of their high-mortality rate. Previous studies have demonstrated the feasibility of using PDI with extracorporeal light to treat pneumonia. In this study, we analyzed key parameters for the viability of this treatment, including the selectivity of the photodynamic response for pathogens over host cells. Our results showed that PDI can induce killing of Staphylococcus aureus (of up to 4.18 log for the strain Xen29 and 3.62 log for Xen36) under conditions where little or no toxicity for host cells is observed. We validated pulmonary delivery of the photosensitizer and light in mice, using photobleaching as an indicator, and demonstrated preservation of healthy tissues as evidence of the safety of the protocol. Overall, PDI displays low toxicity on host tissues, making it a promising tool for treatment of pneumonias caused by S. aureus and other important pathogens.
(© 2020 Wiley-VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: