Estradiol and Progesterone Levels are Related to Redox Status in the Follicular Fluid During in vitro Fertilization.

Autor:	Pizarro BM; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Cordeiro A; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Reginatto MW; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Campos SPC; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Mancebo ACA; Fertipraxis, Clinic of Human Reproduction, Rio de Janeiro, Brazil., Areas PCF; Fertipraxis, Clinic of Human Reproduction, Rio de Janeiro, Brazil., Antunes RA; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Fertipraxis, Clinic of Human Reproduction, Rio de Janeiro, Brazil.; Maternal Hospital of the Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Souza MDCB; Fertipraxis, Clinic of Human Reproduction, Rio de Janeiro, Brazil., Oliveira KJ; Laboratory of Endocrine Physiology and Metabolism, Physiology and Pharmacology, Federal Fluminense University, Niteroi, Brazil., Bloise FF; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Bloise E; Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Fortunato RS; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Ortiga-Carvalho TM; Biophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of the Endocrine Society [J Endocr Soc] 2020 Jun 01; Vol. 4 (7), pp. bvaa064. Date of Electronic Publication: 2020 Jun 01 (Print Publication: 2020).
DOI:	10.1210/jendso/bvaa064
Abstrakt:	Studies have reported a possible association between the levels of oxidative stress biomarkers in follicular fluid (FF) and infertility treatment outcomes. FF analysis can provide important information about oocyte quality. This study aimed to evaluate the possible correlation between oxidative stress biomarker and intrafollicular hormone levels and clinical and laboratory parameters in women during controlled ovarian stimulation. These women were undergoing in vitro fertilization with intracytoplasmic sperm injection (ICSI).The FF samples were acquired from September 2012 to February 2014 from women undergoing private fertility treatment in Rio de Janeiro, Brazil. A total of 196 women who were undergoing ICSI and had different infertility diagnoses were recruited. The FF from each patient (average patient age of 36.3 ± 4.3 years) was collected following puncture of just one follicle with the largest diameter. After ruling out blood contamination by spectrophotometry, 163 patient samples were utilized in the study. In the FF, the progesterone levels were negatively correlated with (a) hydrogen peroxide scavenging capacity (HPSC) ( r = -0.294, P < 0.0001), (b) total number of follicles ( r = -0.246, P < 0.001) and (c) total number of oocytes punctured ( r = -0.268, P = 0.0001). The concentration of serum estradiol exhibited a positive correlation with intrafollicular HPSC ( r = 0.165, P = 0.037). Our data indicate that the FF levels of estradiol and progesterone are related to the FF redox status, which is closely associated with the number of oocytes obtained during ICSI procedures. (© Endocrine Society 2020.)
Databáze:	MEDLINE
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