| Autor: |
Do TBT; Faculty of Engineering and Food Technology, Hue University of Agriculture and Forestry, Hue University, Thua Thien Hue 530000, Vietnam., Tran TAL; Hue Tourism College, Thua Thien Hue 530000, Vietnam., Tran TVT; University of Sciences, Hue University, Thua Thien Hue 530000, Vietnam., Le TH; University of Sciences, Hue University, Thua Thien Hue 530000, Vietnam., Jayasena V; School of Science and Health, Western Sydney University, NSW 2751 Penrith, Australia., Nguyen THC; Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam.; Faculty of Environmental and Chemical Engineering, Duy Tan University, Da Nang 550000, Vietnam., Nguyen CC; Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam.; Faculty of Environmental and Chemical Engineering, Duy Tan University, Da Nang 550000, Vietnam., Kim SY; Department of Materials Science and Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea., Le QV; Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam.; Department of Materials Science and Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea. |
| Abstrakt: |
This study aimed at providing a route towards the production of a novel exopolysaccharide (EPS) from fermented bamboo shoot-isolated Lactobacillus fermentum . A lactic acid bacteria strain, with high EPS production ability, was isolated from fermented bamboo shoots. This strain, R-49757, was identified in the BCCM/LMG Bacteria Collection, Ghent University, Belgium by the phenylalanyl-tRNA synthetase gene sequencing method, and it was named Lb. fermentum MC3. The molecular mass of the EPS measured via gel permeation chromatography was found to be 9.85 × 10 4 Da. Moreover, the monosaccharide composition in the EPS was analyzed by gas chromatography-mass spectrometry. Consequently, the EPS was discovered to be a heteropolysaccharide with the appearance of two main sugars-D-glucose and D-mannose-in the backbone. The results of one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance spectroscopy analyses prove the repeating unit of this polysaccharide to be [→6)-β-D-Glc p -(1→3)-β-D-Man p -(1→6)-β-D-Glc p -(1→] n , which appears to be a new EPS. The obtained results open up an avenue for the production of novel EPSs for biomedical applications. |
