Cell-extrinsic requirement for sulfate in regulating hippocampal neurogenesis.

Autor:	Zhang Z; School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Australia., Jhaveri D; Mater Research Institute, The University of Queensland, TRI Building, Woolloongabba, Brisbane, 4102, Australia.; Queensland Brain Institute, The University of Queensland, Brisbane, 4072, Australia., Sharmin S; School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Australia., Harvey TJ; School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Australia., Dawson PA; Mater Research Institute, The University of Queensland, TRI Building, Woolloongabba, Brisbane, 4102, Australia., Piper M; School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Australia d.simmons@uq.edu.au m.piper@uq.edu.au.; Queensland Brain Institute, The University of Queensland, Brisbane, 4072, Australia., Simmons DG; School of Biomedical Sciences, The University of Queensland, Brisbane, 4072, Australia d.simmons@uq.edu.au m.piper@uq.edu.au.
Jazyk:	angličtina
Zdroj:	Biology open [Biol Open] 2020 Jul 31; Vol. 9 (7). Date of Electronic Publication: 2020 Jul 31.
DOI:	10.1242/bio.053132
Abstrakt:	Sulfate is a key anion required for a range of physiological functions within the brain. These include sulfonation of extracellular proteoglycans to facilitate local growth factor binding and to regulate the shape of morphogen gradients during development. We have previously shown that mice lacking one allele of the sulfate transporter Slc13a4 exhibit reduced sulfate transport into the brain, deficits in social behaviour, reduced performance in learning and memory tasks, and abnormal neurogenesis within the ventricular/subventricular zone lining the lateral ventricles. However, whether these mice have deficits in hippocampal neurogenesis was not addressed. Here, we demonstrate that adult Slc13a4 +/- mice have increased neurogenesis within the subgranular zone (SGZ) of the hippocampal dentate gyrus, with elevated numbers of neural progenitor cells and intermediate progenitors. In contrast, by 12 months of age there were reduced numbers of neural stem cells in the SGZ of heterozygous mice. Importantly, we did not observe any changes in proliferation when we isolated and cultured progenitors in vitro in neurosphere assays, suggestive of a cell-extrinsic requirement for sulfate in regulating hippocampal neurogenesis. Collectively, these data demonstrate a requirement for sulfate transport during postnatal brain development to ensure normal adult hippocampal neurogenesis. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2020. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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