| Autor: |
Celiński K; Department of Genetics, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland., Sokołowska J; Department of Genetics, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland., Zemleduch-Barylska A; Department of Biochemistry and Food Analysis, Faculty of Food and Nutrition Sciences, University of Life Sciences, Mazowiecka 48, 60-623 Poznań, Poland., Kuna R; Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nita, Tr. A. Hlinku 1, 949 74, Nitra, Slovakia., Kijak H; Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland., Staszak AM; Laboratory of Plant Physiology, Department of Plant Biology and Ecology, Faculty of Biology, University of Bialystok, Ciołkowskiego 1J, 15-245 Białystok, Poland., Wojnicka-Półtorak A; Department of Genetics, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland., Chudzińska E; Department of Genetics, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland. |
| Abstrakt: |
The Pinus mugo complex includes several dozen closely related European mountain pines. The discrimination of specific taxa within this complex is still extremely challenging, although numerous methodologies have been used to solve this problem, including morphological and anatomical analyses, cytological studies, allozyme variability, and DNA barcoding, etc. In this study, we used the seed total protein (STP) patterns to search for taxonomically interesting differences among three closely-related pine taxa from the Pinus mugo complex and five more distant species from the Pinaceae family. It was postulated that STP profiling can serve as the backup methodology for modern taxonomic research, in which more sophisticated analyses, i.e., based on the DNA barcoding approach, have been found to be useless. A quantitative analysis of the STP profiles revealed characteristic electrophoretic patterns for all the analyzed taxa from Pinaceae. STP profiling enabled the discrimination of closely-related pine taxa, even of those previously indistinguishable by chloroplast DNA barcodes. The results obtained in this study indicate that STP profiling can be very useful for solving complex taxonomic puzzles. |
