Lichenicoccus roseus gen. nov., sp. nov., the first bacteriochlorophyll a -containing, psychrophilic and acidophilic Acetobacteraceae bacteriobiont of lichen Cladonia species.

Autor: Pankratov TA; S.N. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Grouzdev DS; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Patutina EO; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Kolganova TV; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Berestovskaya JJ; S.N. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia., Ashikhmin AA; Institute of Basic Biological Problems of Russian Academy of Sciences, Federal Research Center, Pushchino Scientific Center for Biological Research of Russian Academy of Sciences, Pushchino 142290, Russia.
Jazyk: angličtina
Zdroj: International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2020 Aug; Vol. 70 (8), pp. 4591-4601. Date of Electronic Publication: 2020 Jul 13.
DOI: 10.1099/ijsem.0.004318
Abstrakt: Gram-negative, aerobic, chemo-organotrophic and bacteriochlorophyll a -containing bacterial strains, KEBCLARHB70R T , KAMCLST3051 and KAMCLST3152, were isolated from the thalli of Cladonia arbuscula and Cladonia stellaris lichens. Cells from the strains were coccoid and reproduced by binary division. They were motile at the early stages of growth and utilized sugars and alcohols. All strains were psychrophilic and acidophilic, capable of growth between pH 3.5 and 7.5 (optimum, pH 5.5), and at 4-30 °C (optimum, 10-15 °C). The major fatty acids were C 18 : 1   ω7 c and C 18 : 0 ; the lipids were phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids, phosphatidylglycerol, glycolipids, diphosphatidylglycerol and polar lipids with an unknown structure. The quinone was Q-10. The DNA G+C content was 67.8 mol%. Comparative 16S rRNA gene analysis together with other data, supported that the strains, KEBCLARHB70R T , KAMCLST3051 and KAMCLST3152 belonged to the same species. Whole genome analysis of the strain KEBCLARHB70R T and average amino acid identity values confirmed its distinctive phylogenetic position within the family Acetobacteraceae . Phenotypic, ecological and genomic characteristics distinguished strains KEBCLARHB70R T , KAMCLST3051 and KAMCLST3152 from all genera in the family Acetobacteraceae . Therefore, we propose a novel genus and a novel species, Lichenicoccus roseus gen. nov., sp. nov., for these novel Acetobacteraceae members. Strain KEBCLARHB70R T (=KCTC 72321 T =VKM B-3305 T ) has been designated as the type strain.
Databáze: MEDLINE