Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy.

Autor: Mohamed EA; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Bordean DM; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Radulov I; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Moruzi RF; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Hulea CI; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Orășan SA; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Dumitrescu E; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Muselin F; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Herman H; 'Vasile Goldis' Western University Arad, Revoluției Blvd. No. 94, Arad, Romania., Brezovan D; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania., Hermenean A; 'Vasile Goldis' Western University Arad, Revoluției Blvd. No. 94, Arad, Romania., Cristina RT; Banat University of Agriculture and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 119 Calea Aradului, Timisoara 300645, Romania.
Jazyk: angličtina
Zdroj: Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2020 Jun 22; Vol. 2020, pp. 1736803. Date of Electronic Publication: 2020 Jun 22 (Print Publication: 2020).
DOI: 10.1155/2020/1736803
Abstrakt: Background: Medications to reduce oxidative stress are preventing cellular damage associated with hyperlipidemia. In this regard, statins (e.g., atorvastatin) act primarily by decrease in low-density lipoprotein- c but, in the last decade, hepatotoxicity, associated with liver injuries in the next months after treatments' initiation, was reported. In this case, associated phytotherapy can be a solution.
Purpose: To investigate the antioxidant potential and response to free radicals, in the case of hyperlipidemic rats treated with atorvastatin. Sea buckthorn ( Hippophae rhamnoides ) and a grape extract (antioxivita) efficiency in the oxidative stress were investigated, also being ascertained the rats' organs cytoarchitecture.
Methods: Eighty-four hyperlipidemic Wistar rats were divided into seven groups and orally treated as follows: ATS, atorvastatin (20 mg/kg·bw); ATS + Hr, atorvastatin +  H. rhamnoides ; ATS + Aox, atorvastatin + grape extract; Hr, H. rhamnoides ; and Aox, grape extract (both as 100 mg/kg·bw). HFD and Control received high fat diet and normal fodder only. After two and six months, respectively, rats were euthanized and the heart, liver, and kidneys were gathered. The tissue samples were prepared by homogenization of 0.5 g tissue, in ethanol, kept for 48 hours at 4°C-10°C and then filtered, in order to assess organs' cytoarchitecture and the TAC's values (by using cupric ion reducing antioxidant capacity (CUPRAC) assay). The test tubes were incubated, at room temperature, for 30 minutes, and then analyzed using a spectrophotometer at 450-650 nm.
Results: The statistics (ANOVA) revealed that sea buckthorn diminished notably ( p < 0.001) the oxidative stress in the heart, liver, and kidney. After six months, the TAC's reduced levels for the heart were significant ( p < 0.001) in ATS + Aox. In the case of histology, the liver's cytoarchitecture in ATS revealed abnormal cytoarchitecture. In ATS + Hr, ATS + Aox, Hr, and Aox, cell regeneration improved in different stages, especially for ATS + Hr and ATS + Aox, in comparison with HFD, which exhibited fat degeneration. Kidney's cytoarchitecture revealed cellular healing, especially in ATS + Hr and ATS + Aox.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2020 Erieg A. Mohamed et al.)
Databáze: MEDLINE
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