| Autor: |
Rivero-Wendt CLG; Universidade Anhanguera-Uniderp, Campo Grande, MS, Brazil., Miranda-Vilela AL; Department of Genetics and Morphology, Institute of Biological Sciences, Universidade de Brasília, Brasília, DF, Brazil., Domingues I; Department of Biology & CESAM, Universidade de Aveiro, Aveiro, Portugal., Oliveira R; Department of Biology & CESAM, Universidade de Aveiro, Aveiro, Portugal., Monteiro MS; Department of Biology & CESAM, Universidade de Aveiro, Aveiro, Portugal., Moura-Mello MAM; Department of Biology & CESAM, Universidade de Aveiro, Aveiro, Portugal., Matias R; Universidade Anhanguera-Uniderp, Campo Grande, MS, Brazil., Soares AMVM; Department of Biology & CESAM, Universidade de Aveiro, Aveiro, Portugal.; Graduate Program in Vegetal Production, Universidade Federal do Tocantins, Gurupi, TO, Brazil., Grisolia CK; Department of Genetics and Morphology, Institute of Biological Sciences, Universidade de Brasília, Brasília, DF, Brazil. |
| Abstrakt: |
The 17 alpha methyltestosterone (MT) hormone is fed to Oreochromis niloticus larvae in fish farms with the purpose of inducing sex reversal. The aim of this study was to evaluate the toxicity and sub-lethality of MT (99.9% purity) and cMT (a commercial MT with 90% purity) in zebrafish ( Danio rerio ) adults, where the animals were exposed to concentrations of 0, 4, 23, 139, 833 and 5000 µg/L for 96 hours. Genotoxicity was evaluated by micronucleus test (MN), nuclear abnormalities (NA) and comet assay. A low genotoxic potential of MT was showed, inducing micronucleus, nuclear abnormalities and DNA damage in Danio rerio , depending on the use of MT or cMT, gender and tested concentrations. In the sub-lethality trials, there was a basal difference in the activity of the enzymatic biochemical markers for males and females, while the Glutatione S transferase (GST) activity decreased in all analyzed tissues, and for males the enzymatic activity decreased only in the intestine. Results suggest that MT has a toxic potential to fish because it alters enzymatic metabolic pathways and may pose a risk to the ecosystems. |
