Mammalian deltavirus without hepadnavirus coinfection in the neotropical rodent Proechimys semispinosus .

Autor:	Paraskevopoulou S; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany., Pirzer F; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany., Goldmann N; Institute of Medical Virology, Justus Liebig University Giessen, 35392 Giessen, Germany.; National Reference Centre for Hepatitis B Viruses and Hepatitis D Viruses, 35392 Giessen, Germany.; German Centre for Infection Research (DZIF), partner site Giessen, 35392 Giessen, Germany., Schmid J; Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, 89069 Ulm, Germany.; Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Republic of Panama., Corman VM; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany., Gottula LT; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany., Schroeder S; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany., Rasche A; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany., Muth D; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany., Drexler JF; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.; Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov University, 119991 Moscow, Russia., Heni AC; Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, 89069 Ulm, Germany.; Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Republic of Panama., Eibner GJ; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, 89069 Ulm, Germany.; Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Republic of Panama., Page RA; Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Republic of Panama., Jones TC; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; Centre for Pathogen Evolution, Department of Zoology, University of Cambridge, CB2 3EJ Cambridge, United Kingdom., Müller MA; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.; Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov University, 119991 Moscow, Russia., Sommer S; Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, 89069 Ulm, Germany; simone.sommer@uni-ulm.de dieter.glebe@viro.med.uni-giessen.de christian.drosten@charite.de., Glebe D; Institute of Medical Virology, Justus Liebig University Giessen, 35392 Giessen, Germany; simone.sommer@uni-ulm.de dieter.glebe@viro.med.uni-giessen.de christian.drosten@charite.de.; National Reference Centre for Hepatitis B Viruses and Hepatitis D Viruses, 35392 Giessen, Germany.; German Centre for Infection Research (DZIF), partner site Giessen, 35392 Giessen, Germany., Drosten C; Institute of Virology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany; simone.sommer@uni-ulm.de dieter.glebe@viro.med.uni-giessen.de christian.drosten@charite.de.; German Centre for Infection Research (DZIF), partner site Charité, 10117 Berlin, Germany.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Jul 28; Vol. 117 (30), pp. 17977-17983. Date of Electronic Publication: 2020 Jul 10.
DOI:	10.1073/pnas.2006750117
Abstrakt:	Hepatitis delta virus (HDV) is a human hepatitis-causing RNA virus, unrelated to any other taxonomic group of RNA viruses. Its occurrence as a satellite virus of hepatitis B virus (HBV) is a singular case in animal virology for which no consensus evolutionary explanation exists. Here we present a mammalian deltavirus that does not occur in humans, identified in the neotropical rodent species Proechimys semispinosus The rodent deltavirus is highly distinct, showing a common ancestor with a recently described deltavirus in snakes. Reverse genetics based on a tandem minus-strand complementary DNA genome copy under the control of a cytomegalovirus (CMV) promoter confirms autonomous genome replication in transfected cells, with initiation of replication from the upstream genome copy. In contrast to HDV, a large delta antigen is not expressed and the farnesylation motif critical for HBV interaction is absent from a genome region that might correspond to a hypothetical rodent large delta antigen. Correspondingly, there is no evidence for coinfection with an HBV-related hepadnavirus based on virus detection and serology in any deltavirus-positive animal. No other coinfecting viruses were detected by RNA sequencing studies of 120 wild-caught animals that could serve as a potential helper virus. The presence of virus in blood and pronounced detection in reproductively active males suggest horizontal transmission linked to competitive behavior. Our study establishes a nonhuman, mammalian deltavirus that occurs as a horizontally transmitted infection, is potentially cleared by immune response, is not focused in the liver, and possibly does not require helper virus coinfection. Competing Interests: The authors declare no competing interest. (Copyright © 2020 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu