| Autor: |
Fibbe WE; Department of Hematology, University Medical Center, Leiden, The Netherlands., Kluck PM, Duinkerken N, Voogt PJ, Willemze R, Falkenburg JH |
| Jazyk: |
angličtina |
| Zdroj: |
European journal of haematology [Eur J Haematol] 1988 Oct; Vol. 41 (4), pp. 352-8. |
| DOI: |
10.1111/j.1600-0609.1988.tb00209.x |
| Abstrakt: |
Mononuclear phagocytes play an important role in the regulation of hematopoiesis, not only by producing regulatory monokines such as prostaglandins, tumor necrosis factor and interleukin-1 (IL-1), but also by the production of colony-stimulating activity (CSA). Previously, we have demonstrated that granulocyte-macrophage CSA (GM-CSA) production by mononuclear phagocytes can be induced by IL-1. In the present study, the influence of culture conditions on the production of GM-CSA was studied. It was found that both human sera and fetal bovine sera contain constituents - at present undefined - that induce GM-CSA production. These factors are distinct from IL-1 and lipopolysaccharide. In selected experiments, no GM-CSA-inducing effect of serum was found, suggesting that the effect may be donor-related. GM-CSA release in the presence of serum could be reduced by 40% after incubation of mononuclear phagocytes at low cell concentrations in methylcellulose, indicating that intimate cell-cell contact is an additional factor that enhances GM-CSA release. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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