Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants.

Autor:	Ren G; Department of Plant Sciences, University of California, Davis, CA, USA.; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China., Rosquete MR; Department of Plant Sciences, University of California, Davis, CA, USA.; Plant Biology Lab. Salk Institute for Biological Studies, La Jolla, CA, USA., Peralta AG; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA., Pattathil S; Mascoma LLC (Lallemand Inc.), Lebanon, NH, USA.; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA., Hahn MG; Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA., Wilkop T; Light Microscopy Core, University of Kentucky, Lexington, KY, USA., Drakakaki G; Department of Plant Sciences, University of California, Davis, CA, USA. gdrakakaki@ucdavis.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2177, pp. 153-167.
DOI:	10.1007/978-1-0716-0767-1_13
Abstrakt:	The dynamic endomembrane system facilitates sorting and transport of diverse cargo. Therefore, it is crucial for plant growth and development. Vesicle proteomic studies have made substantial progress in recent years. In contrast, much less is known about the identity of vesicle compartments that mediate the transport of polysaccharides to and from the plasma membrane and the types of sugars they selectively transport. In this chapter, we provide a detailed description of the protocol used for the elucidation of the SYP61 vesicle population glycome. Our methodology can be easily adapted to perform glycomic studies of a broad variety of plant cell vesicle populations defined via subcellular markers or different treatments.
Databáze:	MEDLINE
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