Cell-free biosensors for rapid detection of water contaminants.

Autor: Jung JK; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.; Center for Water Research, Northwestern University, Evanston, IL, USA., Alam KK; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.; Center for Water Research, Northwestern University, Evanston, IL, USA., Verosloff MS; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.; Center for Water Research, Northwestern University, Evanston, IL, USA.; Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL, USA., Capdevila DA; Department of Chemistry, Indiana University, Bloomington, IN, USA., Desmau M; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL, USA., Clauer PR; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Lee JW; Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Republic of Korea., Nguyen PQ; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA., Pastén PA; Departmento de Ingeniería Hidráulica y Ambiental, Pontificia Universidad Católica de Chile, Santiago, Chile.; Centro de Desarrollo Urbano Sustentable, Santiago, Chile., Matiasek SJ; Department of Geological and Environmental Sciences, California State University, Chico, Chico, CA, USA.; Center for Water and the Environment, California State University, Chico, Chico, CA, USA., Gaillard JF; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL, USA., Giedroc DP; Department of Chemistry, Indiana University, Bloomington, IN, USA.; Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA., Collins JJ; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.; Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, MA, USA.; Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA, USA.; Broad Institute of MIT and Harvard, Cambridge, MA, USA., Lucks JB; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. jblucks@northwestern.edu.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. jblucks@northwestern.edu.; Center for Water Research, Northwestern University, Evanston, IL, USA. jblucks@northwestern.edu.; Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL, USA. jblucks@northwestern.edu.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2020 Dec; Vol. 38 (12), pp. 1451-1459. Date of Electronic Publication: 2020 Jul 06.
DOI: 10.1038/s41587-020-0571-7
Abstrakt: Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA Output Sensors Activated by Ligand Induction (ROSALIND) to detect contaminants in water. A combination of highly processive RNA polymerases, allosteric protein transcription factors and synthetic DNA transcription templates regulates the synthesis of a fluorescence-activating RNA aptamer. The presence of a target contaminant induces the transcription of the aptamer, and a fluorescent signal is produced. We apply ROSALIND to detect a range of water contaminants, including antibiotics, small molecules and metals. We also show that adding RNA circuitry can invert responses, reduce crosstalk and improve sensitivity without protein engineering. The ROSALIND system can be freeze-dried for easy storage and distribution, and we apply it in the field to test municipal water supplies, demonstrating its potential use for monitoring water quality.
Databáze: MEDLINE
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