Adipose-Derived Stromal Cells Seeded on Integra ® Dermal Regeneration Template Improve Post-Burn Wound Reconstruction.

Autor: Piejko M; Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.; Department of General Surgery, Jagiellonian University Medical College, 31-215 Krakow, Poland., Radziun K; Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland., Bobis-Wozowicz S; Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland., Waligórska A; Department of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland., Zimoląg E; Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland., Nessler M; Malopolska Center for Burns and Plastic Surgery, The Ludwik Rydygier Hospital, 31-826 Krakow, Poland., Chrapusta A; Malopolska Center for Burns and Plastic Surgery, The Ludwik Rydygier Hospital, 31-826 Krakow, Poland., Madeja Z; Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland., Drukała J; Cell Bank, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
Jazyk: angličtina
Zdroj: Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2020 Jul 02; Vol. 7 (3). Date of Electronic Publication: 2020 Jul 02.
DOI: 10.3390/bioengineering7030067
Abstrakt: Fibrosis of burn-related wounds remains an unresolved clinical issue that leads to patient disability. The aim of this study was to assess the efficacy of the transplantation of adipose-derived stromal cells seeded onto a collagen-based matrix in the reconstruction of burn-related scars. Here, we characterized an in vitro interaction between adipose-derived stromal cells and a collagen-based matrix, Integra ® DRT. Our results show that transcription of pro-angiogenic, remodeling, and immunomodulatory factors was more significant in adipose-derived stromal cells than in fibroblasts. Transcription of metalloproteinases 2 and 9 is positively correlated with the collagenolytic activity of the adipose-derived stromal cells seeded onto Integra ® DRT. The increase in the enzymatic activity corresponds to the decrease in the elasticity of the whole construct. Finally, we validated the treatment of a post-excision wound using adipose-derived stromal cells and an Integra ® DRT construct in a 25-year-old woman suffering from burn-related scars. Scarless healing was observed in the area treated by adipose-derived stromal cells and the Integra ® DRT construct but not in the reference area where Integra ® DRT was applied without cells. This clinical observation may be explained by in vitro findings: Enhanced transcription of the vascular endothelial growth factor as well as remodeling of the collagen-based matrix decreased mechanical stress. Our experimental treatment demonstrated that the adipose-derived stromal cells seeded onto Integra ® DRT exhibit valuable properties that may improve post-excision wound healing and facilitate skin regeneration without scars.
Databáze: MEDLINE
Externí odkaz: