| Autor: |
Matos R; Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, Portugal.; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal., De Witte C; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, Belgium., Smet A; Translational Research in Immunology and Inflammation, Laboratory of Experimental Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Antwerp University, B-2610 Antwerp, Belgium., Berlamont H; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, Belgium., De Bruyckere S; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, Belgium., Amorim I; Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, Portugal.; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal., Gärtner F; Instituto de Investigação e Inovação em Saúde da Universidade do Porto (i3S), 4200-135 Porto, Portugal.; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 4050-313 Porto, Portugal., Haesebrouck F; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, 9820 Merelbeke, Belgium. |
| Abstrakt: |
A combined agar and broth dilution method followed by qPCR was used to determine the antimicrobial susceptibility of feline H. heilmannii and H. ailurogastricus isolates. All H. ailurogastricus isolates showed a monomodal distribution of MICs for all the antimicrobial agents tested. For H. heilmannii , a bimodal distribution was observed for azithromycin, enrofloxacin, spectinomycin, and lincomycin. Single nucleotide polymorphisms (SNPs) were found in 50S ribosomal proteins L2 and L3 of the H. heilmannii isolate not belonging to the WT population for azithromycin, and in 30S ribosomal proteins S1, S7, and S12 of the isolate not belonging to the WT population for spectinomycin. The antimicrobial resistance mechanism to enrofloxacin and lincomycin remains unknown (2 and 1 H. heilmannii isolate(s), resp.). Furthermore, H. heilmannii isolates showed higher MICs for neomycin compared to H. ailurogastricus isolates which may be related to the presence of SNPs in several 30S and 50S ribosomal protein encoding genes and ribosomal RNA methyltransferase genes. This study shows that acquired resistance to azithromycin, spectinomycin, enrofloxacin, and lincomycin occasionally occurs in feline H. heilmannii isolates. As pets may constitute a source of infection for humans, this should be kept in mind when dealing with a human patient infected with H. heilmannii . |
