First record of native entomopathogenic nematodes from Montana agroecosystems.

Autor: Sandhi RK; Department of Research Centers, Western Triangle Agricultural Research Center, Montana State University , 9546 Old Shelby Rd., P.O. Box 656, Conrad, MT 59425., Pothula R; Department of Biology, Monte L. Bean Museum, and Evolutionary Ecology Laboratories, Brigham Young University , Provo, UT 84602., Pothula SK; Department of Biology, Monte L. Bean Museum, and Evolutionary Ecology Laboratories, Brigham Young University , Provo, UT 84602., Adams BJ; Department of Biology, Monte L. Bean Museum, and Evolutionary Ecology Laboratories, Brigham Young University , Provo, UT 84602., Reddy GVP; Department of Research Centers, Western Triangle Agricultural Research Center, Montana State University , 9546 Old Shelby Rd., P.O. Box 656, Conrad, MT 59425 ; USDA-ARS, Southern Insect Management Research Unit, 141 Experiment Station Road, P.O. Box 346, Stoneville, MS 38776.
Jazyk: angličtina
Zdroj: Journal of nematology [J Nematol] 2020; Vol. 52, pp. 1-11.
DOI: 10.21307/jofnem-2020-060
Abstrakt: A total of 30 different agricultural fields in the Golden Triangle Region of Montana, USA were surveyed, and 150 soil samples were evaluated for the presence of entomopathogenic nematodes (EPNs). The authors isolated EPNs from 10% of the collected samples. The recovered isolates were identified as Steinernema feltiae and Heterorhabditis bacteriophora by using morphological and molecular analysis. Steinernema feltiae was found from two fields, Kalispell ( S. feltiae 1) and Choteau ( S. feltiae 2). Steinernema feltiae (1 and 2) differed significantly from each other in terms of morphological characters for infective juveniles (distance from anterior end to excretory pore and nerve ring) and 1st generation males (body length, spicule length, gubernaculum length, oesophagus, tail, and anal body diameter). Steinernema feltiae 2 and H. bacteriophora were recovered from the same field in Choteau. All these species were recovered from wheat fields with sandy clay loam and loam soils with 3.3 to 3.4% organic matter content and pH 8.
A total of 30 different agricultural fields in the Golden Triangle Region of Montana, USA were surveyed, and 150 soil samples were evaluated for the presence of entomopathogenic nematodes (EPNs). The authors isolated EPNs from 10% of the collected samples. The recovered isolates were identified as Steinernema feltiae and Heterorhabditis bacteriophora by using morphological and molecular analysis. Steinernema feltiae was found from two fields, Kalispell ( S. feltiae 1) and Choteau ( S. feltiae 2). Steinernema feltiae (1 and 2) differed significantly from each other in terms of morphological characters for infective juveniles (distance from anterior end to excretory pore and nerve ring) and 1st generation males (body length, spicule length, gubernaculum length, oesophagus, tail, and anal body diameter). Steinernema feltiae 2 and H. bacteriophora were recovered from the same field in Choteau. All these species were recovered from wheat fields with sandy clay loam and loam soils with 3.3 to 3.4% organic matter content and pH 8.
Databáze: MEDLINE
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