The comparison of pegylated liposomal doxorubicin and beta-carotene effects on JAR and JEG-3 choriocarcinoma human cell culture models

Autor: Erol SA; Clinic of Obstetrics and Gynecology, Ankara City Hospital, Ankara, Turkey, Sel G; Clinic of Obstetrics and Gynecology, Zonguldak Bülent Ecevit University Health Practice and Research Hospital, Zonguldak, Turkey, Harma Mİ; Clinic of Obstetrics and Gynecology, Division of Gynecologic Oncology, Zonguldak Bülent Ecevit University Health Practice and Research Hospital, Zonguldak, Turkey, Harma M; Clinic of Obstetrics and Gynecology, Division of Gynecologic Oncology, Zonguldak Bülent Ecevit University Health Practice and Research Hospital, Zonguldak, Turkey, Tekin İÖ; Clinic of Immunology, Zonguldak Bülent Ecevit University Health Practice and Research Hospital, Zonguldak, Turkey
Jazyk: angličtina
Zdroj: Journal of the Turkish German Gynecological Association [J Turk Ger Gynecol Assoc] 2020 Sep 03; Vol. 21 (3), pp. 171-179. Date of Electronic Publication: 2020 Jul 06.
DOI: 10.4274/jtgga.galenos.2020.2019.0199
Abstrakt: Objective: The aim was to investigate the effectiveness of pegylated liposomal doxorubicin (PLD), beta-carotene, and a combination of PLD and beta-carotene on JAR and JEG-3 human choriocarcinoma (CC) cell lines for the treatment of CC.
Material and Methods: JAR and JEG-3 cells were cultured. PLD and beta-carotene trial groups were determined with different doses (for single drug trial; PLD 1, 2, 5 μg/mL and beta-carotene 1, 5, 10 μg/mL, and for combined drug trial; all PLD doses combined with beta-carotene 5 μg/mL). Drugs were administered to cultures simultaneously, and 72 hours later the cells were detached using trypsin-ethylenediamine tetraacetic acid solution. The percentage of apoptotic cells was determined by flow cytometry after annexin V staining. One set of the supernatant was collected before trypsin application to investigate beta-human chorionic gonadotropin (β-hCG) and hyperglycosylated hCG (H-hCG) levels. Statistical analyses of the apoptotic ratios were performed using Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney U tests.
Results: Apoptosis increased in JAR and JEG-3 cultures after treatment with all doses of PLD (p<0.05). A single application of each betacarotene dose increased apoptosis in JAR cells (p<0.05) but had no apoptotic effects on JEG-3 cells. In the PLD and beta-carotene combination group, apoptosis increased in both JAR and JEG-3 cells (p<0.05).
Conclusion: To our knowledge, this is the first investigation of the effectiveness of PLD, beta-carotene, and PLD + beta-carotene combination therapy in two different CC cell lines. PLD is a promising chemotherapeutic drug, and beta-carotene can be used as a novel non-chemotherapeutic agent for treatment of CC. Based on the results of this study, vitamin A supplementation may have promise as a preventive measure. However, these data need support from animal experiments and clinical trials.
Databáze: MEDLINE
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