Lipid excess affects chaperone-mediated autophagy in hypothalamus.

Autor: Portovedo M; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Reginato A; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Miyamoto JÉ; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Simino LA; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Hakim MP; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Campana M; Université de Paris, BFA, UMR 8251, CNRS, F-75013, Paris, France., Leal RF; IBD Research Laboratory, Colorectal Surgery Unit, Department of Surgery, School of Medical Sciences, UNICAMP, Campinas, 13083-878, Brazil., Ignácio-Souza LM; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Torsoni MA; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Magnan C; Université de Paris, BFA, UMR 8251, CNRS, F-75013, Paris, France., Le Stunff H; Université de Paris, BFA, UMR 8251, CNRS, F-75013, Paris, France., Torsoni AS; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil., Milanski M; Laboratory of Metabolic Disorders, Faculty of Applied Sciences, UNICAMP, Limeira, 13484-350, Brazil. Electronic address: marciane.milanski@fca.unicamp.br.
Jazyk: angličtina
Zdroj: Biochimie [Biochimie] 2020 Sep; Vol. 176, pp. 110-116. Date of Electronic Publication: 2020 Jul 02.
DOI: 10.1016/j.biochi.2020.06.008
Abstrakt: Obesity is a major health problem worldwide. Overweight and obesity directly affect health-related quality of life and also have an important economic impact on healthcare systems. In experimental models, obesity leads to hypothalamic inflammation and loss of metabolic homeostasis. It is known that macroautophagy is decreased in the hypothalamus of obese mice but the role of chaperone-mediated autophagy is still unknown. In this study, we aimed to investigate the role of hypothalamic chaperone-mediated autophagy in response to high-fat diet and also the direct effect of palmitate on hypothalamic neurons. Mice received chow or high-fat diet for 3 days or 1 week. At the end of the experimental protocol, chaperone-mediated autophagy in hypothalamus was investigated, as well as cytokines expression. In other set of experiments, neuronal cell lines were treated with palmitic acid, a saturated fatty acid. We show that chaperone-mediated autophagy is differently regulated in response to high-fat diet intake for 3 days or 1 week. Also, when hypothalamic neurons are directly exposed to palmitate there is activation of chaperone-mediated autophagy. High-fat diet causes hypothalamic inflammation concomitantly to changes in the content of chaperone-mediated autophagy machinery. It remains to be studied the direct role of inflammation and lipids itself on the activation of chaperone-mediated autophagy in the hypothalamus in vivo and also the neuronal implications of chaperone-mediated autophagy inhibition in response to obesity.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
Databáze: MEDLINE
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