The neural stem-cell marker CD24 is specifically upregulated in IDH-mutant glioma.
| Autor: | Tiburcio PDB; Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, UT, USA; Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA., Locke MC; Department of Radiation Oncology, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA., Bhaskara S; Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA; Department of Radiation Oncology, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA., Chandrasekharan MB; Department of Radiation Oncology, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA., Huang LE; Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, UT, USA; Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA. Electronic address: eric.huang@hsc.utah.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Translational oncology [Transl Oncol] 2020 Oct; Vol. 13 (10), pp. 100819. Date of Electronic Publication: 2020 Jul 01. |
| DOI: | 10.1016/j.tranon.2020.100819 |
| Abstrakt: | Background: Malignant gliomas have disproportionally high morbidity and mortality. Heterozygous mutations in the isocitrate dehydrogenase 1 (IDH1) gene are most common in glioma, resulting in predominantly arginine to histidine substitution at codon 132. Because IDH1 R132H requires a wild-type allele to produce (D)-2-hydroxyglutarate for epigenetic reprogramming, loss of IDH1 R132H heterozygosity is associated with glioma progression in an IDH1-wildtype-like phenotype. Although previous studies have reported that transgenic IDH1 R132H induces the expression of nestin-a neural stem-cell marker, the underlying mechanism remains unclear. Furthermore, this finding seems at odds with better outcome of IDH1 R132H glioma because of a negative association of nestin with overall survival. Methods: Gene expression was compared between IDH1 R132H -hemizygous and IDH1 R132H -heterozygous glioma cells under adherent and spheroid growth conditions. The results were validated for (D)-2-hydroxyglutarate responsiveness by pharmacologic agents, associations with DNA methylation by bioinformatic analysis, and associations with overall survival. Bisulfite DNA sequencing, chromatin immunoprecipitation, and pharmacological approach were used. Findings: Neural stem-cell marker genes, including CD44, NES, and PROM1, are generally downregulated in IDH-mutant gliomas and IDH1 R132H -heterozygous spheroid growth compared respectively with IDH-wildtype gliomas and IDH1 R132H -hemizygous spheroid growth, in agreement with their negative associations with patient outcome. In contrast, CD24 is specifically upregulated and apparently associated with better survival. CD24 and NES expression respond differentially to alteration of (D)-2-hydroxyglutarate levels. CD24 upregulation is associated with histone and DNA demethylation as opposed to hypermethylation in the downregulated genes. Interpretation: The better outcome of IDH-mutant glioma is orchestrated exquisitely through epigenetic reprogramming that directs bidirectional expression of neural stem-cell marker genes. Competing Interests: Declaration of competing interest None. (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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