High-throughput nucleotide sequencing for bacteriome studies in oral squamous cell carcinoma: a systematic review.

Autor: Ramos RT; Department of Medical Clinic, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Sodré CS; Department of Medical Clinic, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., de Sousa Rodrigues PMGR; Faculty of Dentistry, Estácio de Sá University (UNESA), Rio de Janeiro, Brazil., da Silva AMP; Faculty of Dentistry, Estácio de Sá University (UNESA), Rio de Janeiro, Brazil., Fuly MS; Faculty of Dentistry, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Dos Santos HF; Department of Marine Biology, Fluminense Federal University (UFF), Rio de Janeiro, Brazil., Gonçalves LS; Faculty of Dentistry, Estácio de Sá University (UNESA), Rio de Janeiro, Brazil. luciogoncalves@yahoo.com.br., de Carvalho Ferreira D; Faculty of Dentistry, Estácio de Sá University (UNESA), Rio de Janeiro, Brazil., Ribeiro MG; Service of Medical Genetics, IPPMG - Martagão Gesteira Pediatric Institute, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Oral and maxillofacial surgery [Oral Maxillofac Surg] 2020 Dec; Vol. 24 (4), pp. 387-401. Date of Electronic Publication: 2020 Jul 03.
DOI: 10.1007/s10006-020-00873-4
Abstrakt: Purpose: Dysbiosis has been identified in oral squamous cell carcinoma (OSCC). The aim of this study was to carry out a systematic review of an electronic research that was carried out on articles published between January 2008 and September 2018.
Methods: Eight studies were selected after applying the inclusion and exclusion criteria.
Results: All articles targeted the hypervariable regions of the 16S rRNA gene. At the phylum level, it was found reduction of Bacteroidetes (2/8 studies) and increase of Firmicutes (2/8 studies). At the genus level, Rothia increased (1/8 studies) and decreased (2/8 studies) in tumor samples, and Streptococcus also was found increased (3/8 studies) and reduced (3/8 studies). Fusobacterium only increased in OSCC samples (3/8 studies). At species level, an increase in F. nucleatum subsp. polymorphum was more associated to OSCC (2/8 studies) than with controls, as was P. aeruginosa (3/8 studies).
Conclusion: In summary, the results corroborated dysbiosis in OSCC patients, with enrichment of microbial taxa that are associated with inflammation and production of acetaldehyde. However, variations of study design and sample size were observed among the studies, as well as a shortage of more detailed analyses of possible correlations between risk habits and OSCC. This lack of more detailed analysis may be the cause of the inconsistencies in regard of the alterations reported for certain genera and species. In conclusion, there is an association between OSCC and oral microbiota dysbiosis, but its role in oral carcinogenesis needs to be clarified in more detail.
Databáze: MEDLINE
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