| Autor: |
Mejia NR; Department of Biochemistry, McGill University, Montreal, Que. Canada., MacKenzie RE |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1988 Aug 30; Vol. 155 (1), pp. 1-6. |
| DOI: |
10.1016/s0006-291x(88)81040-4 |
| Abstrakt: |
Transformed mammalian cells express a unique bifunctional NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase in addition to the usual NADP-dependent dehydrogenase-cyclohydrolase-synthetase. Subcellular fractionation of murine cell lines revealed that the NAD-dependent dehydrogenase activity is located predominantly in mitochondria, while the NADP-dependent trifunctional dehydrogenase appears to exist only in the cytosol of these cells. Western analysis using monospecific polyclonal antisera confirms the subcellular distribution of these two proteins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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