Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus.

Autor:	Yan C; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Liu H; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Jia Y; Department of Animal Engineering, Yangling Vocational & Technical College, Yangling 712100, Shaanxi Province, China., Prince-Theodore DW; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China; Institut Superieur du Developpement Rural (ISDR), Universite de Bangui BP 1450, Bangui, Republique Centrafricaine., Yang M; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Addoma Adam FE; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China; Department of Preventive Medicine and Public Health, Faculty of Veterinary Science, University of Nyala, Nyala, Sudan., Ren J; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Cao X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Wang X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Xiao S; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China., Zhang S; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: zhangshuxia316@163.com., Yang Z; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China. Electronic address: yzq8162@163.com.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2020 Jul; Vol. 99 (7), pp. 3374-3384. Date of Electronic Publication: 2020 May 04.
DOI:	10.1016/j.psj.2020.04.014
Abstrakt:	Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbird is a velogenic virus, and NDV-Dove is a lentogenic virus. Further analysis revealed that the F proteins of the 2 strains were identical, and only 5 amino acid sites on the HN proteins were inconsistent. Five different HN mutant plasmids were constructed and analyzed in this study. The results showed that the mutation F110L caused a significant increase in fusion-promotion activity caused by an increase in neuraminidase activity. Because of the increase in receptor-binding activity caused by G116R, there was a significant increase in fusion-promotion activity. The mutation G54S resulted in a slight decrease in the fusion-promotion activity caused by a slight decrease in receptor-binding activity. The slight increase in the fusion-promotion activity caused by A469V was associated with a significant increase in neuraminidase activity. Therefore, the amino acids L110 and R116 played a key role in determining the virulence difference between NDV-Blackbird and NDV-Dove, which could lay a foundation for illuminating the virulence differences of NDV strains, as well as the development of attenuated vaccines. (Copyright © 2020. Published by Elsevier Inc.)
Databáze:	MEDLINE
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