Detecting bracoviral orthologs distribution in five tsetse fly species and the housefly genomes.

Autor: Kimenyi KM; Centre for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya., Abry MF; Centre for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya., Okeyo W; International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, Kenya., Matovu E; Makerere University, P.O. Box 7062, Kampala, Uganda., Masiga D; International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi, 00100, Kenya., Kulohoma BW; Centre for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya. bkulohoma@uonbi.ac.ke.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2020 Jul 02; Vol. 13 (1), pp. 318. Date of Electronic Publication: 2020 Jul 02.
DOI: 10.1186/s13104-020-05161-8
Abstrakt: Objective: Mutualism between endogenous viruses and eukaryotes is still poorly understood. Several endogenous double-stranded polydnaviruses, bracoviruses, homologous to those present in parasitic braconid wasp genomes were detected in the tsetse fly (Glossina morsitans morsitans). This is peculiar since tsetse flies do not share a reproductive lifestyle similar to wasps, but deliver fully developed larvae that pupate within minutes of exiting their mothers. The objective of this study is to investigate genomic distribution of bracoviral sequences in five tsetse fly species and the housefly, and examine its value as a potential vector control strategy target point. We use comparative genomics to determine the presence, distribution across Glossina species genomes, and evolutionary relationships of bracoviruses of five tsetse fly species and the housefly.
Results: We report on homologous bracoviruses in multiple Dipteran genomes. Phylogenetic reconstruction using within-species concatenated bracoviral orthologs shows great congruence with previously reconstructed insect species phylogenies. Our findings suggest that bracoviruses present in Diptera originate from a single integration event of the viral genome that occurred in an ancestor insect before the evolutionary radiation of different insect orders.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje