Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells.

Autor: Heller L; Department of Medical Engineering, University of South Florida, Tampa, Florida, USA., Thinard R; ALSaTECH, Tufts Biolabs Launchpad, Boston, Massachusetts, USA., Chevalier M; ALSaTECH, Tufts Biolabs Launchpad, Boston, Massachusetts, USA., Arpag S; Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, USA., Jing Y; Center for Bioelectrics, Old Dominion University, Norfolk, Virginia, USA., Greferath R; ALSaTECH, Tufts Biolabs Launchpad, Boston, Massachusetts, USA., Heller R; Department of Medical Engineering, University of South Florida, Tampa, Florida, USA., Nicolau C; ALSaTECH, Tufts Biolabs Launchpad, Boston, Massachusetts, USA.; Friedman School of Nutrition Science and Policy, Tufts University, Boston, Massachusetts, USA.
Jazyk: angličtina
Zdroj: Journal of cellular and molecular medicine [J Cell Mol Med] 2020 Aug; Vol. 24 (15), pp. 8772-8778. Date of Electronic Publication: 2020 Jul 01.
DOI: 10.1111/jcmm.15511
Abstrakt: In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood-brain barrier (BBB) breakdown. After intravenous or intra-arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to promote neurovascular repair. Autologous EPCs transfected to express specific therapeutic proteins offer an innovative therapeutic option. Here, we demonstrate that EPC transfection by electroporation with plasmids encoding the reporter protein GFP or an anti-β-amyloid antibody fragment (Fab) leads to secretion of each protein. We also demonstrate the secreted anti-β-amyloid Fab protein functions in β-amyloid aggregate solubilization.
(© 2020 ALSaTECH. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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