| Autor: |
Livingston DP 3rd; USDA-ARS and North Carolina State University, Raleigh, NC, USA. dpl@ncsu.edu., Tuong TD; USDA-ARS and North Carolina State University, Raleigh, NC, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2156, pp. 119-139. |
| DOI: |
10.1007/978-1-0716-0660-5_10 |
| Abstrakt: |
Histological analysis of frozen and thawed plants has been conducted for many years but the observation of individual sections only provides a two-dimensional representation of a three-dimensional phenomenon. Currently available optical sectioning techniques for viewing internal structures in three dimensions are either low in resolution or the instrument cannot penetrate deep enough into the tissue to visualize the whole plant. Methods using higher resolution equipment are expensive and often require time-consuming training. In addition, conventional stains cannot be used for optical sectioning techniques. We present a relatively simple and less expensive technique using pixel-based (JPEG) images of conventionally stained histological sections of an Arabidopsis thaliana plant. The technique uses commercially available software to generate a 3D representation of internal structures. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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