DNA mini-barcoding of leporids using noninvasive fecal DNA samples and its significance for monitoring an invasive species.

Autor:	Rodrigues NT; Departamento de Genética e Evolução Universidade Federal de São Carlos São Carlos Brazil., Saranholi BH; Departamento de Genética e Evolução Universidade Federal de São Carlos São Carlos Brazil., Angeloni TA; Departamento de Genética e Evolução Universidade Federal de São Carlos São Carlos Brazil., Pasqualotto N; Programa Interunidades de Pós-Graduação em Ecologia Aplicada (PPGI-EA) Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ/USP) e Centro de Energia Nuclear na Agricultura (CENA/USP) Universidade de São Paulo Piracicaba Brazil.; Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Universidade de São Paulo Ribeirão Preto São Paulo Brazil., Chiarello AG; Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Universidade de São Paulo Ribeirão Preto São Paulo Brazil., Galetti PM Jr; Departamento de Genética e Evolução Universidade Federal de São Carlos São Carlos Brazil.
Jazyk:	angličtina
Zdroj:	Ecology and evolution [Ecol Evol] 2020 Jun 05; Vol. 10 (12), pp. 5219-5225. Date of Electronic Publication: 2020 Jun 05 (Print Publication: 2020).
DOI:	10.1002/ece3.5863
Abstrakt:	Introduced in South America at the end of the 19th century, the European hare population has expanded dramatically and now represents a risk to native Brazilian forest rabbits. Monitoring the invasive Lepus europaeus and its coexistence with native Sylvilagus brasiliensis is a challenge that can be efficiently addressed by the use of molecular tools. This work describes a set of primers useful for amplifying three mini-barcodes for the molecular identification of both invasive and native leporid species using degraded fecal DNA. In addition, tests in silico indicate that these mini-barcodes can successfully amplify the DNA sequences of a number of leporids. These mini-barcodes constitute a powerful tool for the monitoring and management of the invasive L. europaeus and the conservation of native rabbits. Competing Interests: There are no competing interests to declare. (© 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu Plný text