In-vitro propagation, callus culture and bioactive lignan production in Phyllanthus tenellus Roxb: a new source of phyllanthin, hypophyllanthin and phyltetralin.

Autor: Nikule HA; Department of Botany, Savitribai Phule Pune University, Pune, 411 007, India.; Central Instrumentation Facility, Savitribai Phule Pune University, Pune, 411 007, India., Nitnaware KM; Department of Botany, Hutatma Rajguru Mahavidyalaya, Rajgurunagar Dist., Pune, 410 505, India., Chambhare MR; Department of Botany, Savitribai Phule Pune University, Pune, 411 007, India., Kadam NS; Department of Botany, Savitribai Phule Pune University, Pune, 411 007, India.; Design Innovation Centre, Department of Chemistry, Savitribai Phule Pune University, Pune, 411 007, India., Borde MY; Department of Botany, Savitribai Phule Pune University, Pune, 411 007, India., Nikam TD; Department of Botany, Savitribai Phule Pune University, Pune, 411 007, India. tdnikam@unipune.ac.in.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Jun 30; Vol. 10 (1), pp. 10668. Date of Electronic Publication: 2020 Jun 30.
DOI: 10.1038/s41598-020-67637-8
Abstrakt: This is the first report on identification and quantification of important hepatoprotective and anticancer polyphenolic lignans such as phyllanthin (PH), hypophyllanthin (HPH), niranthin (NH) and phyltetralin (PT) in natural plant and in vitro cultures of Phyllanthus tenellus Roxb. The identification of lignans was carried out by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) and quantified using High-Performance Liquid Chromatography (HPLC). In addition, an efficient protocol has been developed for multiple shoot induction in nodal explants of in vitro derived shoots of P. tenellus. Maximum number of shoot regeneration (7.83 ± 0.15) was achieved on medium incorporated with 1.0 mg/l 6-Benzylaminopurine (BAP). The medium containing Indole-3-acetic acid (IAA) 2 mg/l was superior for induction of rooting in in vitro raised shoots. The plantlets were acclimatized to the field condition with 100% survival. The quantitative HPLC analysis showed that the lignan content was variable with the auxins and cytokinins incorporated in the medium. The lignan content was higher in callus grown on Murashige and Skoog (MS) medium + 2.0 mg/l Naphthaleneacetic acid (NAA). The reported protocol can be used for mass propagation and application of biotechnological approaches for improvement of P. tenellus. The results indicate intriguing possibilities for the utilization of P. tenellus plant parts as an alternative source and of callus culture to scale up bioactive lignan production for pharmaceutical applications.
Databáze: MEDLINE
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