Na + transporter HKT1;2 reduces flower Na + content and considerably mitigates the decline in tomato fruit yields under saline conditions.

Autor: Romero-Aranda MR; Department of Plant Breeding and Biotechnology, La Mayora Institute for Mediterranean and Subtropical Horticulture, UMA/CSIC, Malaga, Spain., González-Fernández P; Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/ Prof. Albareda 1, 18008, Granada, Spain., Pérez-Tienda JR; Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/ Prof. Albareda 1, 18008, Granada, Spain., López-Diaz MR; Department of Plant Breeding and Biotechnology, La Mayora Institute for Mediterranean and Subtropical Horticulture, UMA/CSIC, Malaga, Spain., Espinosa J; Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/ Prof. Albareda 1, 18008, Granada, Spain., Granum E; Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/ Prof. Albareda 1, 18008, Granada, Spain., Traverso JÁ; Department of Cellular Biology, Faculty of Sciences, University of Granada, 18071, Granada, Spain., Pineda B; Laboratory of Tissue Culture and Plant Breeding, Institute of Plant Molecular and Cellular Biology, Polytechnic University of Valencia-CSIC, Valencia, 46022, Spain., Garcia-Sogo B; Laboratory of Tissue Culture and Plant Breeding, Institute of Plant Molecular and Cellular Biology, Polytechnic University of Valencia-CSIC, Valencia, 46022, Spain., Moreno V; Laboratory of Tissue Culture and Plant Breeding, Institute of Plant Molecular and Cellular Biology, Polytechnic University of Valencia-CSIC, Valencia, 46022, Spain., Asins MJ; Plant Protection and Biotechnology Center, Valencian Institute of Agrarian Research (IVIA), 46113, Moncada, Valencia, Spain., Belver A; Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), C/ Prof. Albareda 1, 18008, Granada, Spain. Electronic address: andres.belver@eez.csic.es.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2020 Sep; Vol. 154, pp. 341-352. Date of Electronic Publication: 2020 May 24.
DOI: 10.1016/j.plaphy.2020.05.012
Abstrakt: Genes encoding HKT1-like Na + transporters play a key role in the salinity tolerance mechanism in Arabidopsis and other plant species by retrieving Na + from the xylem of different organs and tissues. In this study, we investigated the role of two HKT1;2 allelic variants in tomato salt tolerance in relation to vegetative growth and fruit yield in plants subjected to salt treatment in a commercial greenhouse under real production conditions. We used two near-isogenic lines (NILs), homozygous for either the Solanum lycopersicum (NIL17) or S. cheesmaniae (NIL14) allele, at HKT1;2 loci and their respective RNAi-Sl/ScHKT1;2 lines. The results obtained show that both ScHKT1;2- and SlHKT1;2-silenced lines display hypersensitivity to salinity associated with an altered leaf Na + /K + ratio, thus confirming that HKT1;2 plays an important role in Na + homeostasis and salinity tolerance in tomato. Both silenced lines also showed Na + over-accumulation and a slight, but significant, reduction in K + content in the flower tissues of salt-treated plants and consequently a higher Na + /K + ratio as compared to the respective unsilenced lines. This altered Na + /K + ratio in flower tissues is associated with a sharp reduction in fruit yield, measured as total fresh weight and number of fruits, in both silenced lines under salinity conditions. Our findings demonstrate that Na + transporter HKT1;2 protects the flower against Na + toxicity and mitigates the reduction in tomato fruit yield under salinity conditions.
(Copyright © 2020 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: