| Autor: |
Dos Santos Leandro E; Department of Nutrition, College of Health Sciences, University of Brasília, Brasília, DF, 70910900, Brazil. eliananutunb@gmail.com., Ginani VC; Department of Nutrition, College of Health Sciences, University of Brasília, Brasília, DF, 70910900, Brazil., de Alencar ER; College of Agronomy and Veterinary Medicine, University of Brasília, Brasília, DF, 70910900, Brazil., Pereira OG; Department of Animal Science, Federal University of Viçosa, Viçosa, MG, 36570-000, Brazil., Rose ECP; Department of Nutrition, College of Health Sciences, University of Brasília, Brasília, DF, 70910900, Brazil., do Vale HMM; Department of Phytopathology, University of Brasília, Brasília, DF, 70910900, Brazil., Pratesi R; Interdisciplinary Laboratory of Biociences, Faculty of Medicine, University of Brasília, Brasília, DF, 70910900, Brazil., Hecht MM; Interdisciplinary Laboratory of Biociences, Faculty of Medicine, University of Brasília, Brasília, DF, 70910900, Brazil., Cavalcanti MH; Department of Nutrition, College of Health Sciences, University of Brasília, Brasília, DF, 70910900, Brazil., Tavares CSO; Department of Nutrition, College of Health Sciences, University of Brasília, Brasília, DF, 70910900, Brazil. |
| Abstrakt: |
The objective of this study was to isolate and characterize lactic acid bacteria with probiotic potential in silages of different species of forage plants, cocoa beans, and artisanal salami. The obtained isolates were submitted to the following evaluations: (i) screening for tolerance to pH 2 and bile salts, (ii) genotypic identification of isolates, (iii) survival in simulated gastric and pancreatic conditions, (iv) antimicrobial activity, (v) antibiotic susceptibility and safety, and (vi) properties associated with adhesion capacity. A total of 82 isolates were obtained and were screened for pH 2.0 tolerance and capacity to growth in the presence of bile salts (1.0 and 2.0%). Only 19 strains simultaneously presented tolerance to pH 2.0 and bile salts. These 19 strains were evaluated for genetic profile by Box-PCR. Subsequently, the selected strains were subjected to partial sequencing of the 16S rRNA gene. The species Lactobacillus plantarum was prevalent. The identified strains were evaluated for survival under simulated gastric and pancreatic conditions. Some strains have shown tolerance in both conditions. Different strains showed variations in antimicrobial activity, susceptibility to antibiotics, and properties associated with adhesion (hydrophobicity, autoaggregation, coaggregation, and adhesion to CaCo2 cells). All strains were negative for hemolysis, DNase, gelatinase, and biogenic amine synthesis activity. The L. plantarum SBR64.7 strain can be considered the most promising for it presented the lowest viability reduction when exposed to gastric and pancreatic juices. |
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