Bumblebee Workers Show Differences in Allele-Specific DNA Methylation and Allele-Specific Expression.

Autor:	Marshall H; Department of Genetics and Genome Biology, University of Leicester, United Kingdom., Jones ARC; Department of Genetics and Genome Biology, University of Leicester, United Kingdom., Lonsdale ZN; Department of Genetics and Genome Biology, University of Leicester, United Kingdom., Mallon EB; Department of Genetics and Genome Biology, University of Leicester, United Kingdom.
Jazyk:	angličtina
Zdroj:	Genome biology and evolution [Genome Biol Evol] 2020 Aug 01; Vol. 12 (8), pp. 1471-1481.
DOI:	10.1093/gbe/evaa132
Abstrakt:	Allele-specific expression is when one allele of a gene shows higher levels of expression compared with the other allele, in a diploid organism. Recent work has identified allele-specific expression in a number of Hymenopteran species. However, the molecular mechanism which drives this allelic expression bias remains unknown. In mammals, DNA methylation is often associated with genes which show allele-specific expression. DNA methylation systems have been described in species of Hymenoptera, providing a candidate mechanism. Using previously generated RNA-Seq and whole-genome bisulfite sequencing from reproductive and sterile bumblebee (Bombus terrestris) workers, we have identified genome-wide allele-specific expression and allele-specific DNA methylation. The majority of genes displaying allele-specific expression are common between reproductive and sterile workers and the proportion of allele-specific expression bias generally varies between genetically distinct colonies. We have also identified genome-wide allele-specific DNA methylation patterns in both reproductive and sterile workers, with reproductive workers showing significantly more genes with allele-specific methylation. Finally, there is no significant overlap between genes showing allele-specific expression and allele-specific methylation. These results indicate that cis-acting DNA methylation does not directly drive genome-wide allele-specific expression in this species. (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)
Databáze:	MEDLINE
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