Bacillus subtilis cardiolipin protects its own membrane against surfactin-induced permeabilization.

Autor: Pinkas D; Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic., Fišer R; Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic. Electronic address: fiserr@natur.cuni.cz., Kozlík P; Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 8, 128 00 Prague, Czech Republic., Dolejšová T; Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic., Hryzáková K; Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic., Konopásek I; Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic., Mikušová G; Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic. Electronic address: seydlova@natur.cuni.cz.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2020 Oct 01; Vol. 1862 (10), pp. 183405. Date of Electronic Publication: 2020 Jun 25.
DOI: 10.1016/j.bbamem.2020.183405
Abstrakt: Surfactin, a cyclic lipoheptapeptide produced by Bacillus subtilis, is a surface-active antimicrobial that targets the barrier function of lipid membranes. It inserts itself into the membrane, where it forms conductive pores. Depending on its concentration, it eventually disintegrates the membrane in a detergent-like manner. The molecular details of this activity are not yet sufficiently understood, nor are the mechanisms that the surfactin producer employs to resist its own toxic product. We have previously shown that B. subtilis modifies its membrane lipid composition upon the onset of surfactin production, mainly increasing the cardiolipin content. Here we show that the increased cardiolipin content leads to a decreased surfactin-induced leakage of liposomes reconstituted from lipids isolated from the surfactin producer. This stabilizing effect of cardiolipin is concentration-dependent. Using a propidium iodide-based cell permeabilization assay, we further confirmed that the cytoplasmic membrane of the mutant B. subtilis strain lacking cardiolipin was substantially more susceptible to the action of surfactin, even though the amount of bound surfactin was the same as in the wild-type strain. We propose that membrane remodelling; due to the increase in cardiolipin content, contributes to the surfactin tolerance of B. subtilis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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