| Autor: |
Paknejad H; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran., Hosseini Shekarabi SP; Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. hosseini.pezhman@yahoo.com., Shamsaie Mehrgan M; Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. m.shamsaie@srbiau.ac.ir., Hajimoradloo A; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran., Khorshidi Z; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran., Rastegari S; Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. |
| Abstrakt: |
Peppermint is a popular herbal medicine due to its several pharmaceutical applications. In this study, peppermint powder was used as a feed additive to evaluate growth performance, hematological parameters, protein profile of skin mucus, and immune parameters, as well as growth hormone (GH), insulin-like growth factor (IGF), and 70 kDa heat shock protein (HSP70) gene expression in Caspian roach (Rutilus caspicus). The fingerlings (average weight of 2.40 ± 0.12 g) were fed with diet containing 0 (control), 2, 3, and 4 g/kg peppermint for 8 weeks. The addition of peppermint significantly enhanced the growth parameters and decreased the food conversion ratio. Hematological indices of fish fed with peppermint-supplemented diets were significantly different from the control group (P < 0.05). Soluble protein, alkaline phosphatase, and lysozyme enzyme activity in mucus samples showed an incremental trend by increasing the peppermint levels in the diet. Evaluation of mucosal immunity indicated a remarkable difference between the protein profile in treatments fed with peppermint-supplemented diets and the control group. A new protein band (approximately 27 kDa) was also found in the skin mucus of fish fed with the diet containing 4 g/kg peppermint, and the highest band density was observed in this treatment. The highest IGF and GH gene expression were observed in 4 g/kg peppermint treatment. There was a significant difference in HSP70 expression between the fish fed with peppermint powder and the control group (P < 0.05). Overall, the results showed that dietary oral administration of peppermint at 4 g/kg of feed can act as a growth promoter and immunostimulant. |
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