Aberrant Cytoplasmic Terminal Deoxynucleotidyl Transferase (TdT) Immunostaining on Previously Frozen, Formalin-fixed Paraffin-embedded Tissue: Cryotomy-induced Antigen Diffusion (CIAD).
| Autor: | Henwood AF; Histopathology Department, The Children's Hospital at Westmead.; School of Medicine, University of Western Sydney, Sydney, Australia. |
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| Jazyk: | angličtina |
| Zdroj: | Applied immunohistochemistry & molecular morphology : AIMM [Appl Immunohistochem Mol Morphol] 2021 Feb 01; Vol. 29 (2), pp. e17-e19. |
| DOI: | 10.1097/PAI.0000000000000863 |
| Abstrakt: | Terminal deoxynucleotidyl transferase (TdT) has been localized in both the nuclear and cytoplasmic compartments of lymphoblastic lymphomas when such tumors have been frozen for cryotomy and subsequently, formalin fixed and processed to paraffin. This cryotomy-induced antigen diffusion (CIAD) occurs with several different sources of anti-TdT antisera and was not observed in nonlymphoblastic tumors. CIAD was also not observed with other nuclear antigens including BRG1, Pax5, Ki67, BCL6, INI-1, myogenin, MyoD1, and Phox2B. TdT CIAD seems to be specific for lymphoblastic lymphomas. CIAD is another preanalytical artifact that needs to be considered in immunohistochemistry. Competing Interests: The author declares no conflict of interest. (Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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