Participation of TDP1 in the repair of formaldehyde-induced DNA-protein cross-links in chicken DT40 cells.

Autor: Nakano T; DNA Damage Chemistry Research Group, Institute for Quantum Life Science, Kansai Photon Science Institute, National Institutes of Quantum and Radiological Science and Technology, Kizugawa, Kyoto, Japan.; Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan., Shoulkamy MI; Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan., Tsuda M; Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan.; Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan., Sasanuma H; Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan., Hirota K; Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan., Takata M; Department of Late Effects Studies, Radiation Biology Center, Kyoto University, Kyoto, Japan., Masunaga SI; Division of Radiation Life Science, Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Osaka, Japan., Takeda S; Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan., Ide H; Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan., Bessho T; The Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, United States of America., Tano K; Division of Radiation Life Science, Institute for Integrated Radiation and Nuclear Science, Kyoto University, Kumatori, Osaka, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Jun 26; Vol. 15 (6), pp. e0234859. Date of Electronic Publication: 2020 Jun 26 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0234859
Abstrakt: Proteins are covalently trapped on DNA to form DNA-protein cross-links (DPCs) when cells are exposed to DNA-damaging agents. Aldehyde compounds produce common types of DPCs that contain proteins in an undisrupted DNA strand. Tyrosyl-DNA phosphodiesterase 1 (TDP1) repairs topoisomerase 1 (TOPO1) that is trapped at the 3'-end of DNA. In the present study, we examined the contribution of TDP1 to the repair of formaldehyde-induced DPCs using a reverse genetic strategy with chicken DT40 cells. The results obtained showed that cells deficient in TDP1 were sensitive to formaldehyde. The removal of formaldehyde-induced DPCs was slower in tdp1-deficient cells than in wild type cells. We also found that formaldehyde did not produce trapped TOPO1, indicating that trapped TOPO1 was not a primary cytotoxic DNA lesion that was generated by formaldehyde and repaired by TDP1. The formaldehyde treatment resulted in the accumulation of chromosomal breakages that were more prominent in tdp1-deficient cells than in wild type cells. Therefore, TDP1 plays a critical role in the repair of formaldehyde-induced DPCs that are distinct from trapped TOPO1.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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