Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines.

Autor: Jeremiasse B; Department of Rheumatology & Clinical Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands., Matta C; Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary. matta.csaba@med.unideb.hu., Fellows CR; Department of Veterinary Pre-Clinical Sciences, School of Veterinary Science and Medicine, University of Surrey, Guildford, UK., Boocock DJ; John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, NG11 8NS, UK., Smith JR; Bruker UK Limited, Coventry, UK., Liddell S; Exonate Ltd., Medicity, Thane Road, Nottingham, UK., Lafeber F; Department of Rheumatology & Clinical Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands., van Spil WE; Department of Rheumatology & Clinical Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands., Mobasheri A; Department of Rheumatology & Clinical Immunology, University Medical Centre Utrecht, Utrecht, The Netherlands. ali.mobasheri@oulu.fi.; Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland. ali.mobasheri@oulu.fi.; Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania. ali.mobasheri@oulu.fi.; Centre for Sport, Exercise and Osteoarthritis Research Versus Arthritis, Queen's Medical Centre, Nottingham, UK. ali.mobasheri@oulu.fi.; Department of Orthopedics, UMC Utrecht, Utrecht, The Netherlands. ali.mobasheri@oulu.fi.
Jazyk: angličtina
Zdroj: BMC molecular and cell biology [BMC Mol Cell Biol] 2020 Jun 26; Vol. 21 (1), pp. 47. Date of Electronic Publication: 2020 Jun 26.
DOI: 10.1186/s12860-020-00288-9
Abstrakt: Background: Chondrocytes are exposed to an inflammatory micro-environment in the extracellular matrix (ECM) of articular cartilage in joint diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). In OA, degenerative changes and low-grade inflammation within the joint transform the behaviour and metabolism of chondrocytes, disturb the balance between ECM synthesis and degradation, and alter the osmolality and ionic composition of the micro-environment. We hypothesize that chondrocytes adjust their physiology to the inflammatory microenvironment by modulating the expression of cell surface proteins, collectively referred to as the 'surfaceome'. Therefore, the aim of this study was to characterize the surfaceome of primary equine chondrocytes isolated from healthy joints following exposure to the pro-inflammatory cytokines interleukin-1-beta (IL-1β) and tumour necrosis factor-alpha (TNF-α). We employed combined methodology that we recently developed for investigating the surfaceome in stem cells. Membrane proteins were isolated using an aminooxy-biotinylation technique and analysed by mass spectrometry using high throughput shotgun proteomics. Selected proteins were validated by western blotting.
Results: Amongst the 431 unique cell surface proteins identified, a high percentage of low-abundance proteins, such as ion channels, receptors and transporter molecules were detected. Data are available via ProteomeXchange with identifier PXD014773. A high number of proteins exhibited different expression patterns following chondrocyte stimulation with pro-inflammatory cytokines. Low density lipoprotein related protein 1 (LPR-1), thrombospondin-1 (TSP-1), voltage dependent anion channel (VDAC) 1-2 and annexin A1 were considered to be of special interest and were analysed further by western blotting.
Conclusions: Our results provide, for the first time, a repository for proteomic data on differentially expressed low-abundance membrane proteins on the surface of chondrocytes in response to pro-inflammatory stimuli.
Databáze: MEDLINE
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